HPLC-DAD-MS法定量分析黄芩中3种黄酮类化合物  

ANALYSIS OF THREE FLAVONOIDS IN SCUTELLARIA BAICALENSIS GEORGI BY HPLC-DAD-MS

作　　者：徐杨璐 赵胜男[1] 申川川 李守拙[1] 

机构地区：[1]河北省中药研究与开发重点实验室,承德医学院中药研究所,河北承德067000

出　　处：《承德医学院学报》2014年第6期461-463,共3页Journal of Chengde Medical University

摘　　要：目的:分析比较高效液相色谱-二极管阵列检测器-质谱(HPLC-DAD-MS)法和高效液相色谱-二极管阵列检测器(HPLC-DAD)法检测黄芩中3种黄酮类化合物的结果。方法:分别采用HPLC-DAD-MS法和HPLC-DAD法检测黄芩中3种黄酮类化合物黄芩苷、汉黄芩苷、汉黄芩素的含量。结果:HPLC-DAD-MS法测得黄芩苷、汉黄芩苷、汉黄芩素的含量分别比HPLC-DAD法平均少6.61%、73.98%和10.90%,其中汉黄芩苷的含量相差较大。结论:与HPLC-DAD法相比,HPLC-DAD-MS法含量测定结果更准确,且操作简便、重现性好,可用于黄芩中黄芩苷、汉黄芩苷、汉黄芩素的含量测定。Objective：To compare the determination of three lfavonoids inScutellaria baicalensis Georgi by high performance liquid chromatography with diode array detection and mass spectrometer （HPLC-DAD-MS） and high performance liquid chromatography with diode array detection （HPLC-DAD）.Methods： HPLC-DAD-MS and HPLC-DAD were respectively applied to determineBaicalin,Wogonoside and Wogonin inScutellaria baicalensis Georgi. Results：The content ofBaicalin, Wogonoside, Wogonin by HPLC-DAD-MS were 6.61%, 73.98% and 10.90% less than HPLC-DAD. Among them, the difference ofWogonoside content was fairly larger.Conclusions： Compared with HPLC-DAD, HPLC-DAD-MS is more accurate, simple and convenient. So HPLC-DAD-MS can be used for the determination of Baicalin, Wogonoside, WogonininScutellaria baicalensis Georgi.

关 键 词：黄芩 高效液相色谱-二极管阵列检测器-质谱法 质量控制 

分 类 号：R284.1[医药卫生—中药学]