中国芒(Miscanthus sinensis)初级核心种质的构建  

Establishment of China's Miscanthus sinensis Primary Core Collection

作　　者：肖亮[1] 易自力[2] 蒋建雄[2] 刘树玲[2] 覃静萍[2] 伊俊奇 杨塞[2] 

机构地区：[1]湖南农业大学作物种质和资源利用国家重点实验室培育基地,长沙410128 [2]湖南农业大学生物科学技术学院,长沙410128

出　　处：《植物遗传资源学报》2014年第6期1196-1201,共6页Journal of Plant Genetic Resources

基　　金：湖南省自然科学基金(12JJ2019);"十二五"农村领域国家科技计划课题(2013BAD22B01)

摘　　要：以555份芒(Miscanthus sinensis)种质资源为研究对象,根据26个表型性状数据,按地理来源、植物区系和单一性状进行分组,分别采用简单比例法、平方根法和多样性指数法确定组内取样数,再根据聚类和随机2种方法进行组内个体选择。依照上述方案共构建出19个具有代表性的芒初选核心种质样本库。通过平均相似系数、性状符合度、数量性状变异系数和遗传多样性指数等4项检测指标对上述19种构建方案进行比较,最终确定了按"植物区划分组+多样性指数确定取样数+聚类选择个体"为芒初级核心种质构建的最佳方案。通过此方法建立起的芒初级核心种质资源共83份,占总资源的14.95%,且新构建的初级种质资源与总资源性状符合度达到100%。In this study,555 Miscanthus sinensis germplasm resources were selected as material.Based on 26 phenotypic traits,19 sampling strategies were used,which consisted of 3 stratification methods such as resource of geographical origin,floristic region and single character,3 sampling number determination methods such as proportioned,square root and diversity index and 2 individual selection methods such as random and cluster.The criteria against whole collection included mean similarity coefficient,coincidence of traits,mean coefficient of variation of quantitaive traits and mean shannon index.The results indicated that an optimal strategy was set by stratification according to floristic region determination of the sampling number with genetic diversity-dependent strategy and selecting sample by clusters.By using this strategy,a pre-core collection of Chinese Miscanthus sinensis germplam with a total of 83 accessions was established,carrying 100% phenotypic genetic diversity of the whole collection.

关 键 词：芒 表型性状 核心种质 遗传多样性指数 

分 类 号：S578[农业科学—作物学]