小麦品系5R618抗叶锈病基因的初步定位  

Genetic Analysis of Leaf Rust Resistance Gene in Wheat Line 5R618

作　　者：王佳真[1] 李在峰[1] 李星[1] 刘大群[1] 

机构地区：[1]河北农业大学植物病理系/河北省农作物病虫害生物防治工程技术研究中心,保定071001

出　　处：《植物遗传资源学报》2014年第6期1348-1351,共4页Journal of Plant Genetic Resources

基　　金：河北省教育厅优秀青年项目(YQ2013024);国家自然科学基金(31361140367)

摘　　要：5R618是高抗叶锈病小麦品系。为了确定该品系所携带的抗叶锈基因,以5R618与感病小麦品种郑州5389杂交获得F1,自交获得F2分离群体以及F2∶3家系,用叶锈菌生理小种THJP对亲本、F2分离群体以及F2∶3家系进行叶锈抗性鉴定,然后进行分子标记分析。结果显示,5R618对生理小种THJP的抗病性由1对显性基因控制,该基因暂命名为Lr5R。经过亲本和抗感池间分子标记筛选以及F2∶3家系的标记检测,Lr5R定位于染色体3DL上,barc71和STS24-16是Lr5R最近的2个标记,遗传距离分别为0.9 c M和2.1 c M。The wheat（Triticum aestivum L.） line 5R618 showed low infection types to most of Chinese current leaf rust pathotypes in the seeding stage.In order to identify the resistance genes in 5R618,F2 segregating populations and F2∶ 3families from a cross between 5R618 and Zhengzhou 5389（susceptible to leaf rust） were inoculated with pathotype THJP in greenhouse.The infection types were investigated after inoculation for 15 d and molecular markers were also used for mapping the resistance genes.The results indicated that 5R618 carried a single dominant resistance gene,temporarily designated as Lr5 R.Using molecular marker methods,Lr5 R was located on chromosome3 DL and closely linked to barc71 and STS24-16,with genetic distances of 0.9 cM and 2.1 cM,respectively.

关 键 词：小麦抗叶锈病基因 遗传分析 分子标记 

分 类 号：S512.1[农业科学—作物学]