水稻白叶枯病拮抗菌的筛选、鉴定及发酵液稳定性分析  

Screening and identification of antagonistic microorganism against Xanthomonas oryzae and stability analysis of the fermentation broth

作　　者：张艳军[1] 张萍华[1] 

机构地区：[1]浙江师范大学化学与生命科学学院,浙江金华321004

出　　处：《浙江师范大学学报（自然科学版）》2014年第4期382-387,共6页Journal of Zhejiang Normal University:Natural Sciences

基　　金：浙江省重中之重学科"现代农业生物技术与作物病害防控"开放基金资助项目

摘　　要：从稻田土壤、水稻植株、昆虫肠道等环境中分离纯化得到88株菌株,以水稻白叶枯病菌生理小种P6菌株为指示菌,通过琼脂块法和牛津杯法从中筛选得到了对水稻白叶枯病菌具有明显拮抗作用的12号菌株,其抑菌圈直径为6.09 cm.根据12号拮抗菌株菌落和菌丝形态特征、生理生化特征及16S r DNA序列分析结果,鉴定该菌株为Streptomyces gramineus.该拮抗菌发酵液具有较强的热稳定性、酸碱稳定性和光照稳定性,将其作为生物农药具有一定的应用价值和开发前景.Rice bacterial leaf blight had been one of the three major diseases in rice,therefore how to effectively control and reduce its damage was the object of a prolonged endeavor of scientific research. 88 strains were separated and purified from rice soil,rice plants and the insect gut. The strain No. 12 which had the strong antagonism on Xanthomonas oryzae P6 was isolated from these strains by agar block method and cylinder-plate method,and the inhibitory zone diameter was 6. 09 cm. According to the morphology,physiological,biochemical tests and 16 S rDNA results,the strain No. 12 was identification as Streptomyces gramineus. The fermentation broth of the strain No. 12 had better stability to heat,acid,alkali,UV light and natural light.Therefore antimicrobial active component produced by the strain No. 12 would have certain application value and development prospects as a biopesticide.

关 键 词：水稻 白叶枯病 拮抗菌 抑菌稳定性 

分 类 号：Q939.96[生物学—微生物学]