高效液相色谱法测定一次性使用真空采血管中的EDTA-Na_2  

HPLC determination of EDTA-Na_2 in disposable vacuum vessel

作　　者：秦黎 尹菁[2] 

机构地区：[1]江苏省医疗器械检验所,南京210033 [2]江苏省食品药品检验所,南京210008

出　　处：《中国医疗器械信息》2014年第11期32-34,共3页China Medical Device Information

摘　　要：目的:建立一次性使用真空采血管中的EDTA-Na2的高效液相色谱检测方法。方法:采用Venusil MP C18柱(250mm×4.6mm,5μm),流动相为甲醇-25%四丁基氢氧化铵-88%甲酸-水(20:1.3:0.6:980)(p H3.5);检测波长:254nm;柱温:30℃;流速:0.6 m L/m i n。结果:乙二胺四乙酸二钠峰不受干扰,在4μg^100μg/m l浓度范围内线性关系良好(r=1.0000)。高、中、低3种浓度的平均回收率为97.4%。结论:本方法快速简便,专属性强,重复性好,结果准确可靠,适用于多种一次性使用真空采血管中EDTA-Na2的检测。Objective： To establish a HPLC method for the quantitative analysis of EDTA-Na： in disposable vacuum vessel. Method： The chromatographic analysis were carried out on a Venusil MP C18 column （250mm×4.6mm, 5μm）, and the mobile phase was methanol-25% tetrebutyl ammonium hydroxide-88% formic acid and water （20：1.3：0.6：980）（pH3.5）. The detection was carried out at 254nm, with a flow rate of 0.6 ml/min. The column temperature was 30℃. Result： Disodium EDTA curves were linear in the range of 4μg-100μg/ml （r=1.0000）, the average recovery was 97.4%. Conclusion： This rapid and simple method shows high specificity and reproducibility, which is suitable for the assay of EDTA-Na2 in disposable vacuum vessel. The results are accurate and reliable.

关 键 词：乙二胺四乙酸二钠盐 一次性使用真空采血管 高效液相色谱法 

分 类 号：R446.1[医药卫生—诊断学]