原发性干燥综合征并发非霍奇金淋巴瘤患者唇腺组织BCL-6基因重排的检测及意义  

Detection and significance of BCL-6 gene rearrangement in salivary glands of patients with primary Sj?gren syndrome combined with non-hodgkin lymphoma

作　　者：杨敏[1] 梁胜翔 李宝贞[1] 石宇红[1] 许佳[1] 莫汉有[1] 

机构地区：[1]桂林医学院附属医院风湿免疫科,桂林541001 [2]空军空降兵学院门诊部,桂林541003

出　　处：《中国当代医药》2014年第34期47-49,共3页China Modern Medicine

基　　金：广西壮族自治区卫生厅课题(Z2010303);广西壮族自治区桂林市科学研究与技术开发计划科技攻关项目(20130120-14)

摘　　要：目的探讨原发性干燥综合征(p SS)并发非霍奇金淋巴瘤(NHL)患者唇腺组织BCL-6基因重排的临床病理意义。方法将研究对象分为p SS并发NHL组(16例),p SS未并发NHL组(20例),NHL未并发p SS组(阳性对照组,20例),健康人群组(阴性对照组,20例),采用荧光原位杂交技术检测4组研究对象唇腺组织BCL-6基因的重排情况。结果 p SS并发NHL组的BCL-6基因重排发生率为56.2%(9/16),p SS未并发NHL组的BCL-6基因重排发生率为20.0%(4/20),NHL未并发p SS组的BCL-6基因重排发生率为50.0%(10/20),正常人群组的BCL-6基因重排发生率为5.0%(1/20)。4组BCL-6基因重排发生率比较差异有统计学意义(χ2=84.225,P=0.000)。p SS并发NHL组与NHL未并发p SS组BCL-6基因重排发生率比较差异无统计学意义(χ2=2.251,P=0.139)。p SS并发NHL组BCL-6基因重排发生率显著高于p SS未并发NHL组(χ2=78.658,P=0.000)及健康人群组(χ2=88.391,P=0.000)。结论 BCL-6基因重排与p SS并发NHL相关,BCL-6基因重排可作为预测p SS并发NHL的生物学参考指标。Objective To explore the clinicopathologic significance of BCL-6 gene rearrangement in salivary glands of patients with primary Sjogren syndrome（pSS）combined with non-hodgkin lymphoma（NHL）. Methods Participants were divided into pSS combined with NHL group （16 eases）,pSS without complication of NHL group （20 cases）,NHL without complication of pSS group （positive control group,20 cases）and healthy group （negative control group,20 eases）.Fluores- cence in situ hybridization was adopted to detect the gene rearrangement of four groups. Results The incidence rate of BCL-6 gene rearrangement in pSS combined with NHL group was 56.2%（9/16）,pSS without complication of NHL group was 20.0%（4/20）,NHL without complication of pSS group was 50.0%（10/20）,while the health group was 5.0%（1/20）.The incidence rate of BCL-6 gene rearrangement in four groups was compared,with statistical difference （χ2=84.225,P= 0.000）.The incidence rate of BCL-6 gene rearrangement in pSS combined with NHL group and NHL without complica- tion of pSS group was compared,with no statistical difference （χ2=2.251,P=0.139）.The incidence rate of BCL-6 gene rearrangement in pSS combined with NHL group was significantly higher than that in pSS without complication of NHL group（χ2=78.658,P=0.000）and health group（χ2=88.391,P=0.000） respectively. Conclusion BCL-6 gene rearrangement is closely related to pSS combined with NHL,whieh can serve as a biological reference index in early diagnosis of pSS combined with NHL.

关 键 词：原发性干燥综合征 非霍奇金淋巴瘤 BCL-6基因 基因重排 荧光原位杂交 

分 类 号：R593[医药卫生—内科学]