用SMMC-7721和Hep-G2全二维细胞膜-整体柱色谱联用技术筛选中药鸦胆子的抗癌活性物质  

Screening of potential active anti-cancer components of Brucea javanica by SMMC-7721 and Hep-G2 comprehensive two dimensional CMC-monolith chromatography

作　　者：纪松岗[1] 丁璇[2] 曹岩[2] 陈啸飞[2] 贾丹[2] 王冬尧[2] 柴逸峰[2] 

机构地区：[1]解放军401医院药剂科,山东青岛266071 [2]第二军医大学药学院,上海200433

出　　处：《药学实践杂志》2014年第6期425-427,433,共4页Journal of Pharmaceutical Practice

基　　金：国家自然科学基金青年项目(12401900802)

摘　　要：目的筛选中药鸦胆子中的抗癌活性成分。方法同时使用全二维色谱技术和细胞膜色谱技术联用质谱技术。结果经初步分离筛选,得到了腺苷和鸦胆子苦素B两种潜在的抗癌活性成分。结论该方法对中药中活性成分的筛选,具有快速、高效和在线的特色和优点。Objective To screen potential active anti-cancer components of Brucea javanica.Methods This research has em-ployed comprehensive two dimensional chromatographic technology and cell membrane chromatographic technology simultaneously with mass spectrometry as detector .Results Adenosine and Bruceine B were found to be potentially anti-cancer active .Conclusion This study has combined the advantages of online , high speed and high throughput for the screen of potential active components of traditional Chinese medicine .

关 键 词：细胞膜色谱 全二维色谱 SMMC-7721 Hep-G2 鸦胆子 抗癌 

分 类 号：O657.7[理学—分析化学]