磷酸肌酸钠对离体大鼠肝脏ALT/LDH指标的影响  

The protective effects of sodium creatine phosphate on the livers' ALT / LDH of cold preservation in vitro in rats

作　　者：苑晓烨[1] 王淼[2] 曹经琳[3] 曾强[3] 窦剑[3] 

机构地区：[1]河北省人民医院老年病二科,石家庄市050051 [2]河北省邢台市人民医院 [3]河北医科大学第三医院肝胆外科

出　　处：《河北医药》2014年第24期3696-3698,共3页Hebei Medical Journal

基　　金：河北省省级重大医学科研课题(编号:zd2013031)

摘　　要：目的观察磷酸肌酸钠(sodium creatine phosphate,SCP)对离体大鼠肝脏中ALT/LDH指标变化,探讨磷酸肌酸在肝脏保护中的重要作用。方法选用Wister系雄性大鼠40只,普通级。应用大鼠肝脏单纯冷保存离体灌注模型,将40只大鼠随机分为对照组(单纯UW液灌注肝脏)、实验组以UW液为基液加入磷酸肌酸钠,依浓度分为高、中、低剂量组(浓度分别1 g/100 ml、2 g/100 ml、3 g/100 ml)。测定不同时间点保存液中丙氨酸氨基转移酶(ALT)及乳酸脱氢酶(LDH)活性。结果实验组在冷保存12 h后,ALT及LDH均低于对照组(P<0.05),随着保存时间的延长,高、中、低剂量组的变化较对照组减轻,各剂量之间无差别(P>0.05)。高剂量实验组在经过24 h冷保存后,肝组织保存液中ALT含量高于对照组及中、低剂量组(P<0.05)。结论磷酸肌酸钠明显降低离体大鼠肝脏中ALT/LDH指标,对离体大鼠肝脏冷保存有较好的保护作用。Objective To explore the protective effect of sodium phosphocreatine on rat livers ’ ALT/LDH of cold preservation , with the objective of providing an effective measure for clinical applications .Methods Simple cold preservation from rat liver perfusion model , Application of the experimental group with high , medium and low three different doses of sodium creatine phosphate UW solution and cryopreserved liver perfusion , the control group simplex UW liver perfusion and cryopreservation.Preservation solution was measured at different time points in alanine aminotransferase （ALT） and lactate dehydrogenase （LDH） activity.Result Different doses of the experimental group after 12h in cold storage,ALT and LDH were lower than the control group .After high dose experimental group after 24h cold preservation,ALT levels were higher in the low dose group.But the experiment found that high doses of the experimental group after group C after 24h cold preservation, ALT levels were higher and the A , B group,considering the drug concentration is too large ,increasing the length time cold preservation of the liver tissue destruction .Conclusions Sodium Phosphocreatine can decreas liver ’ ALT/LDH of cold preservation Significantly ,which has a good protective effect on isolated liver of cold preservation .

关 键 词：离体大鼠肝脏 ALT LDH 磷酸肌酸钠 

分 类 号：R657.3[医药卫生—外科学]