首乌益智胶囊对脑缺血再灌注大鼠学习记忆和海马β淀粉样前体蛋白、早老素1 mRNA表达的影响  

Effects of Shouwu-Yizhi capsule on learning and memory and the expressions of presenilin 1,β-amyloid precursor protein mRNAs in the hippocampus following cerebral ischemia reperfusion in rats

作　　者：陈超[1] 李长生[1] 杨晓妮[1] 程广清[1] 

机构地区：[1]山东省千佛山医院中医科,济南250014

出　　处：《国际中医中药杂志》2014年第12期1096-1099,共4页International Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金（30873323）;国家青年科学基金（81302940）;山东省中医药科技发展计划项目（2013ZDZK-091）

摘　　要：目的：探讨首乌益智胶囊对脑缺血再灌注大鼠学习记忆和早老素1（PS1）、β淀粉样前体蛋白（APP）mRNA表达的影响。方法80只大鼠按体质量随机分为假手术组、模型组、首乌益智胶囊组、脑复康组，每组20只。采用大脑中动脉闭塞法制作脑缺血再灌模型。造模后7 d，首乌益智胶囊组和脑复康组分别给予首乌益智胶囊溶液（52 mg/ml）、脑复康溶液（28 mg/ml）灌胃，均为1 ml/（100 g·d），共28 d，模型组和假手术组等体积生理盐水灌胃。采用Morris水迷宫评价学习和记忆；采用实时荧光定量PCR检测大鼠海马PS1和APP mRNA表达。结果水迷宫实验显示，模型组大鼠逃避潜伏期较假手术组[（12.98±0.70）s比（9.43±0.78）s]显著延长，穿越平台次数较假手术组[（5.08±0.39）次比（7.62±0.43）次]显著减少，首乌益智胶囊组逃避潜伏期较模型组[（9.77±0.58）s比（12.98±0.70）s]显著缩短（P均＜0.01），穿越平台次数较模型组[（7.40±0.44）次比（5.08±0.39）次]显著增加（P均＜0.01）。首乌益智胶囊组海马PS1和APP mRNA 表达[（0.99±0.01）比（1.08±0.03）]均较模型组[（1.06±0.03）比（1.12±0.04）]显著降低（P＜0.05或0.01）。结论首乌益智胶囊可抑制脑缺血再灌大鼠海马PS1和APP mRNA表达，改善学习和记忆。Objective To investigate the effects of Shouwu-Yizhi capsule on learning and memory, and the expressions of presenilin 1（PS1）,β-amyloid precursor protein （APP） mRNAs in the hippocampus following cerebral ischemia reperfusion in rats. Methods Sprague–Dawley rats were randomly divided into a Shouwu-Yizhi group, a piracetam group, a model group, and a sham operation group with 20 rats in each group. Focal cerebral ischemia reperfusion model was induced by middle cerebral artery occlusion for 2 hours. Seven days after ischemia reperfusion, the rats in the Shouwu-Yizhi and piracetam groups were administered intragastrically Shouwu-Yizhi solution （52 mg/ml） and piracetam solution （28 mg/ml） for 28 days, both in dose of 1 ml/（100 g·d） for 28 days;and the rats in the model and sham operation groups were given intragastrically equivalent volumes of normal saline. Learning and memory were tested using the Morris water maze, and the expressions of PS1 and APP mRNAs were measured using real-time quantitative fluorescent PCR. Results Water maze test showed that the escape latency （12.98±0.70s vs. 9.43±0.78s） was significantly shorter, and the frequency of crossing platform （5.08±0.39 vs. 7.62±0.43） significantly lower in the model groupthan those in the sham operation group; the escape latency （9.77±0.58s vs. 12.98±0.70s） was significantly shorter and the frequency of crossing platform （7.40±0.44 vs. 5.08±0.39） significantly lower in the Shouwu-Yizhi group than those in the model group（all P〈0.01）. The expressions of PS1 （0.99±0.01 vs. 1.08± 0.03）and APP （1.06±0.03 vs. 1.12±0.04） mRNAs in the hippocampus in the Shouwu-Yizhi group were significantly decreased than those in the model group （P＆lt;0.05 or 0.01）. Conclusion Shouwu-Yizhi capsule may inhibit the expressions of PS1 and APP mRNAs in the hippocampus, and improve the learning and memory following cerebral ischemia reperfusion in rats.

关 键 词：脑缺血 早老素-1 淀粉样Β蛋白前体 基因表达 学习 记忆 首乌益智胶囊 

分 类 号：R285.5[医药卫生—中药学]