人瘢痕疙瘩成纤维细胞裸鼠荷瘤模型的建立  

Establishment of a keloid model in nude mice with human keloid-derived fibroblasts

作　　者：朱莲花[1] 万红双 金明姬[2] 方宇辉[1] 李周娜[1] 金哲虎[1] 高钟镐[2] 

机构地区：[1]延边大学附属医院皮肤科,吉林延吉133001 [2]中国医学科学院药物研究所

出　　处：《中华皮肤科杂志》2014年第12期845-848,共4页Chinese Journal of Dermatology

基　　金：国家自然科学基金（81260233、81373342）;2013年吉林省产业技术研究与开发专项项目计划（2013C034）;吉林省教育厅“十二五”科技研究项目;吉林省卫生厅科研课题（2011Z085）;北京市自然科学基金（2141004）

摘　　要：目的 建立一种简单、成功率高的人瘢痕疙瘩裸鼠荷瘤模型.方法 27只雌性BALB/c裸鼠随机分为5组,A、B、C组每组5只,在每只裸鼠腋窝下接种人瘢痕疙瘩成纤维细胞和Matrigel胶混悬液0.1 ml,细胞浓度分别为1.0×10^4个/μl Matrigel胶（A组）、3.0×10^4个/μl Matrigel胶（B组）、5.0×10^4个/μl Matrigel胶（C组）.取C组成形瘤块修剪成若干个5 mm×5 mm×5 mm大小的组织块移植于D组裸鼠（8只）的腋窝皮下;E组裸鼠（4只）皮下注射100μl Matrigel胶作为对照组.A、B、C组出瘤后30 d、D组瘤块移植后30 d肉眼观察瘤体的形成过程及变化,并在第31天处死裸鼠进行组织病理学检查,分析其移植后成形的瘤体组织学变化及裸鼠的心、肝、脾、肺、肾组织的变化.结果 A、B、C组裸鼠成瘤率为100％,出瘤时间分别为（90.20±3.96）d、（61.00±2.92）d、（39.60±3.20）d.出瘤30 d时3组瘤体体积分别为（288.34±25.29） mm3、（1 370.63±105.24） mm3、（1 940.98±184.37） mm3,3组差异有统计学意义（F=138.74,P＜ 0.05）.D组裸鼠成形瘤块移植后瘤块体积先略增大后逐渐减小,移植第14天始持续增大,8只中7只成瘤.E组裸鼠未见瘤体形成.组织病理学检查,各组瘤体的组织形态在镜下一致,与人瘢痕疙瘩组织相似,未见其他脏器组织学改变及转移瘤灶.结论 裸鼠皮下接种人瘢痕疙瘩成纤维细胞可建立瘢痕疙瘩裸鼠荷瘤模型,而且已成瘤组织修剪成一定体积再次移植于裸鼠皮下也可以建立瘢痕疙瘩裸鼠荷瘤模型.Objective To establish a simple and efficient method for developing a keloid model in nude mice with human keloid-derived fibroblasts.Methods Twenty-seven female BALB/c nude mice were randomly divided into five groups with 5,5,5,8 and 4 mice in group A,B,C,D and E respectively.The mice in group A,B and C were inoculated with 0.1 ml of suspension containing human keloid-derived fibroblasts at concentrations of 1.0 × 10^4,3.0 × 10^4 and 5.0 × 10^4 per microliter Matrigel,respectively,at the right axillary fossa.The tumors that formed in one mouse in group C were taken out,and cut into several parts measuring 5 mm × 5 mm × 5 mm in size,which were then subcutaneously transplanted into the right axillary fossa of mice in group D.The mice in group E were subcutaneously injected with 100 μl of Matrigel and served as the control group.The formation of tumor in mice was observed by naked eyes,and the size of tumors was measured until day 30 after tumor formation in group A,B and C as well as after tumor transplantation in group D.Mice were sacrificed on day 30 after tumor formation,and histopathologic examination was performed to analyze histological features of transplanted tumors and pathological changes in visceral organs such as heart,liver,spleen,lung and kidney.Results The tumor formation rate was consistently 100% in group A,B and C,and the time required for tumor formation was （90.20 ± 3.96）,（61.00 ± 2.92） and （39.60 ± 3.20） days in group A,B and C respectively.There was a significant difference in tumor volume on the 30th day after tumor formation between group A,B and C （（288.34 ± 25.29） vs.（1 370.63 ± 105.24） vs.（1 940.98 ± 184.37） mm3,F =138.74,P 〈 0.05）.The size of implanted tumor mass in group D firstly increased,then gradually decreased,but began to continuously increase since the 14~ day,and tumor finally formed in 7 out of 8 mice.There was no evidence of tumor formation in group E.Histopathologic examination showed uniform histological manifestations,which were si

关 键 词：瘢痕疙瘩 成纤维细胞 疾病模型 动物 

分 类 号：R622[医药卫生—整形外科] R-332[医药卫生—外科学]