HSP70-2基因多态性与糖尿病肾病易感性的关系  

Heat shock protein 70-2 gene polymorphism and susceptibility to diabetic nephropathy

作　　者：林新伟[1] 杨阳[2] 

机构地区：[1]平顶山市解放军152医院肾内科,河南平顶山467000 [2]郑州大学公共卫生学院,郑州450001

出　　处：《解放军医学杂志》2014年第11期898-901,共4页Medical Journal of Chinese People's Liberation Army

摘　　要：目的探讨HSP70-2(+1267A/G)基因多态性与2型糖尿病肾病的关系。方法收集河南省平顶山市152医院肾内科住院及行定期健康体检的人群资料,按照纳入与排除标准纳入研究对象共677例,其中2型糖尿病患者447例(糖尿病肾病组226例,糖尿病非肾病组221例),正常对照组230例。对研究对象进行一般情况问卷调查、体格检查及实验室生化指标检测,并采用PCR-RFLP技术分析HSP70-2基因(+1267A/G)多态性。结果糖尿病肾病组的HSP70-2基因G/G基因型频率高于糖尿病非肾病组和正常对照组,差异有统计学意义(χ2=8.123,P<0.01;χ2=11.651,P<0.01);糖尿病肾病组的G等位基因频率高于糖尿病非肾病组和正常对照组,差异有统计学意义(χ2=9.392,P<0.01,OR=1.782;χ2=11.971,P<0.01,OR=2.153)。G/G基因型携带者MALB/Cr和BMI水平高于A/A基因携带者,差异有统计学意义(P<0.05)。结论 HSP70-2基因1267位点G/G基因型可能与糖尿病肾病及全身性肥胖易感性相关;G等位基因增加了携带者患糖尿病肾病的风险。Objective To investigate the relationship between heat shock protein 70-2（HSP70-2） gene polymorphism and diabetic nephropathy（DN） in patients with type 2 diabetes mellitus（T2DM）.Methods Following the inclusion and exclusion criteria,a total of 677 subjects were enrolled in present study.They were either hospitalized or undergoing regular health check-up in the Department of Nephrology of PLA 152 nd Hospital in Pingdingshan of Henan Province.Among them 226 patients were suffering from diabetes mellitus and nephrosis（DN＋ group）,221 patients with T2 DM but without nephrosis（DN– group）,and 230 were normal controls（NC group）.Data were collected by questionnaire,physical examination and laboratory examination,and polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP） technique was applied to detect the single nucleotide polymorphisms of HSP70-2（＋1267/G）.Results The G/G genotype frequency of HSP70-2 was significantly higher in DN＋ group than in DN– and NC group（χ2=8.123,P〈0.01;χ2=11.651,P〈0.01）;the G allele frequency of HSP70-2 was significantly higher in DN＋ group than in DN – and NC group（χ2=9.392,P〈0.01,OR=1.782;χ2=11.971,P〈0.01,OR=2.153）.The levels of MALB/Cr and BMI were significantly higher in G/G gene carriers than in A/A gene carriers（P〈0.05）.Conclusion The G/G genotype of HSP70-2（＋1267） may be related to susceptibility to DN and general obesity;G allele carrier may have increased risk of T2 DM with DN.

关 键 词：糖尿病肾病 HSP70热休克蛋白质类 多态性 单核苷酸 

分 类 号：R587.24[医药卫生—内分泌]