不同强度正弦交变电磁场对大鼠骨密度及骨形态计量学的影响  

Effect of different-intensity SEMFs on bone mineral density and histomorphometry in SD rats

作　　者：高玉海[1] 成魁[1] 葛宝丰[1] 甄平[1] 周建[1] 马小妮[1] 李少锋[1] 陈克明[1] 

机构地区：[1]兰州军区兰州总医院骨科研究所,甘肃兰州730050

出　　处：《中国骨伤》2014年第11期933-937,共5页China Journal of Orthopaedics and Traumatology

基　　金：国家自然科学基金(编号:81270963);甘肃省科技重大专项计划资助项目(编号:092NKDA025)~~

摘　　要：目的 :研究50 Hz不同强度正弦交变电磁场(sinusoidal electromagnetic fields,SEMFs)对SD大鼠骨密度和骨形态计量学的影响。方法:将30只雌性SD大鼠随机分为3组:A组(正常对照组),B组(0.1 m T磁场组)和C组(0.6 m T磁场组)。除对照组外,分别给予各磁场组每日3 h的磁场干预。实验8周后处死大鼠,用双能X线骨密度仪检测各组大鼠全身、股骨及椎体骨密度,并对左侧胫骨及椎体L4分别进行骨组织静态和动态分析评价。结果:与A组相比,B组大鼠全身、股骨和椎体骨密度显著增加;股骨和椎体骨小梁面积百分数、骨小梁数量和骨小梁宽度显著增加,骨小梁分离度明显降低;观察双标记有明显变化。C组骨小梁数目与A组相比差异有统计学意义,其余指标差异均无统计学意义。结论:50 Hz 0.1 m T磁场强度的正弦交变电磁场,能够有效增加大鼠骨密度,改善大鼠的骨组织微结构,促进骨形成。Objective：To investigate the effects of different intensity sinusoidal electromagnetic fields（SEMFs） on bone mineral density（BMD） and histomorphometry in SD rats. Methods：Thirty female SD rats were randomly divided into three groups：group A（a control group）,group B（0.1 m T group） and group C（0.6 m T group）. The rats in group B and C were exposed to 50 Hz SEMFs 3 hours each day. However,the magnetic intensity was different between group B and group C ：0.1 m T for group B and 0.6 m T for group C. After 8 weeks,all the animals were killed. Changes of BMD and histomorphometric properties were observed. Results：Compared with group A,the BMD of whole body,femur and vertebrae of rats in group B increased significantly;the area percentage,number and width of bone trabeculae in vertebrae and femur of rats in group B were larger than those of group A;but the resolution of bone trabeculae of rats in group B was lower than that of group A. The trabecular number in group C rats were significantly decreased,compared with that in group A rats. The outcome of double fluorescence labeling in group B was found to be significantly different with that in group A. But the difference between rats in group A and C was not significant. Conclusion：This study demonstrates that 50 Hz 0.1 m T SEMFs can increase BMD,improve bone tissue microstructure and,promote bone formation.

关 键 词：电磁场 骨密度 骨和骨组织 大鼠 

分 类 号：R681[医药卫生—骨科学]