聚多巴胺修饰钛表面纳米载银及其抗菌和细胞相容性  

Bioinspired Polydopamine Functionalization of Titanium Surface for Silver Nanoparticles Immobilization with Antibacterial Property

作　　者：谭英[1] 谭帼馨[1] 宁成云[2] 容锡沧[3] 张余[3] 周蕾[1] 

机构地区：[1]广东工业大学轻工化工学院,广州510006 [2]华南理工大学材料科学与工程学院,广州510641 [3]广州军区总医院骨科医院,广州510010

出　　处：《无机材料学报》2014年第12期1320-1326,共7页Journal of Inorganic Materials

基　　金：国家973计划项目(2012CB619100);国家自然科学基金(51372087;81271957)~~

摘　　要：通过多巴胺自聚合在钛表面构建了仿生聚多巴胺(PDA)膜层,有利于类骨羟基磷灰石在钛表面的沉积,体现了良好的生物活性。利用聚多巴胺的螯合效应及还原性,将纳米银颗粒载入聚多巴胺修饰钛表面;利用场发射扫描电镜(FE-SEM)、X射线光电子能谱(XPS)、显微激光拉曼光谱(Raman)和石墨炉原子吸收光谱(GF-AA5)对聚多巴胺/纳米银修饰钛表面的银粒径、含量及离子释放进行表征。采用杀菌率和表面细菌粘附对聚多巴胺/纳米银修饰钛表面的体外抗菌性能进行检测,研究结果表明:纳米银对金黄色葡萄球菌具有较强的杀菌能力,且MC3T3-E1细胞早期粘附和增殖结果证实本研究得到的聚多巴胺/纳米银修饰钛表面具有良好的体外细胞相容性。A bioinspired polydopamine （PDA） layer was deposited on titanium surface by simply dipping the sub- strate into an alkaiine dopamine solution. The in vitro bioactivity of the polydopamine coated titanium was assessed by incubation in simulated body fluids （SBF）. The results showed that surface-anchored catecholamine moieties in polydopamine enriched the interface with calcium ions, facilitating the deposition of hydroxyapatite. Silver nanoparticles （AgNPs） were metallised on the PDA-grafted titanium surface in mild aqueous environments. About 14% silver release as Ag＋ ions were found after 5 d when the surfaces were exposed to deionized water. The AgNPs showed an efficient microbicidal activity against Staphylococcus Aureus bacterial strains, while not significantly affecting osteoblast cells （MC3T3-E1） viability.

关 键 词：钛 聚多巴胺 纳米银颗粒 抗菌性能 细胞相容性 

分 类 号：TB331[一般工业技术—材料科学与工程]