摘除卵巢对母山羊肾周脂肪中脂肪酸组成及相关基因mRNA表达量的影响  

Effects of Ovariectomy on Fatty Acid Composition and m RNA Expression of Its Related Genes in Perirenal Adipose from Female Goat (Capra hircus)

作　　者：张磊[1] 王燕燕[1] 周占琴[1] 付明哲[1] 李广[1] 安宁[1] 张锁良 尹海科 王忠林 张胜刚 任宝华 

机构地区：[1]西北农林科技大学动物科技学院,杨凌712100 [2]陕西省布尔山羊繁育中心,麟游721500

出　　处：《农业生物技术学报》2014年第12期1544-1552,共9页Journal of Agricultural Biotechnology

基　　金：国家现代肉羊产业技术体系(No.nycytx-39)

摘　　要：公羊去势(摘除睾丸)作为一项十分成熟的生产技术,在畜牧生产上得到了非常广泛的应用,但是有关母山羊(Capra hircus)去势的研究还不多。本研究旨在探讨去势对母山羊血清中血糖、甘油三酯、总胆固醇水平,肾周脂肪组织中脂肪酸的组成以及对乙酰Co A羧化酶(acetly-Co A carboxylase,ACC)、脂肪酸合成酶(fatty acid synthase,FAS)、脂蛋白酯酶(lipoprotein lipase,LPL)和激素敏感酯酶(hormone sensitive lipase,HSL)基因表达的影响。将体重相近的5月龄母山羊摘除卵巢后测定其血糖、甘油三酯、胆固醇水平;采用Agilent-6890N气相色谱仪测定其肾周脂肪组织中脂肪酸的组成;利用实时荧光定量PCR(q RT-PCR)相对定量方法检测FAS、ACC、LPL和HSL m RNA的相对表达量。结果表明,摘除卵巢后,母山羊血清中血糖、甘油三酯和总胆固醇水平较对照组均无显著性差异(P>0.05),而且在整个实验过程中保持稳定;肾周脂肪组织中总饱和脂肪酸和4种饱和脂肪酸(SFA)(癸酸(C10∶0)、十五碳酸(C15∶0)、棕榈酸(C16∶0)和木质素酸(C24∶0))的比例(P<0.05或P<0.01)显著降低;肾周脂肪中总单不饱和脂肪酸(MUFA)和总多不饱和脂肪酸(PUFA)的比例与对照组差异不显著(P>0.05),但是表现出了一种上升的趋势,油酸(C18∶1)比例显著上升(P<0.05);脂肪酸代谢相关基因中LPL m RNA表达量显著提高(P<0.05),ACC和FAS基因的表达量极显著提高(P<0.01),HSL基因的表达量显著降低(P<0.05)。摘除卵巢不会打破母山羊血清中血糖、甘油三酯、总胆固醇的平衡,可能通过影响肾周脂肪组织中脂肪酸代谢相关基因(FAS,ACC,LPL和SHL)m RNA的表达,降低肾周脂肪中饱和脂肪酸的比例,有利于改善羊肉的风味。本研究结果为揭示摘除母山羊卵巢改善肾周脂肪中脂肪酸的构成提供基础资料。Castration has been used in livestock production widely as a mature technology, but little information is available about the effects of ovariectomy on the fatty acid composition which is regulated by lipid-metabolic genes and would potentially improve mutton flavor. The aim of this study was to explore theeffects of ovariectomy on the levels of blood sugar, triglyceride and total cholesterol in serum, the fatty acid composition and fatty acid metabolism related genes expression in perirenal fat. Hybrid kid of Boer goat （Capra hircus） with similar body weight at about 5-month old were treated as the experimental animals to determine the levels of blood sugar, triglyceride and total cholesterol. Then the Agilent- 6890N gas chromatograph was used to determine the composition of fatty acids and Real-time quantitative PCR （qRT-PCR） was used to detect the expression levels of acetly-CoA carboxylase （ACC）, fatty acid synthase （FAS）, lipoprotein lipase （LPL） and hormone sensitive lipase （HSL） genes in perirenal adipose. There was no statistical significant differences on the levels of blood sugar, triglyceride and total cholesterol in serum between Ovx and Con groups, and remained stable in the process of the whole test （P〉0.05）. Significant decreases of the percentage of total saturated fatty acid （SFA） and 4 kinds of SFA （decanoic acid （C10∶0）, pentadecanoic acid （C15∶0）, palmitic acid （C16∶0） and lignoceric acid （C24∶0）） were observed in perirenal fat of ovariectomized goats compared with controls （P〈0.05 or P〈0.01）. The differences of total mono unsaturated fatty acids （MUFA） and polyunsaturated fatty acid （PUFA） between 2 groups were not significant, with rising trend in MUFA （P〉0.05）, and the percentage of oleic acid （C18∶1） was significantly higher in Ovx group than that in Con group （P〈0.05）. The relative expression mRNA levels of fatty acid metabolism related genes were corrected by glyceraldehyde-3-phosphatedehydrog

关 键 词：卵巢摘除 母山羊 肾周脂肪 脂肪酸 MRNA 

分 类 号：S851.347[农业科学—预防兽医学]