基于DNA条形码的百合属分子鉴定  

Molecular identification in genus of Lilium based on DNA barcoding

作　　者：郑司浩[1] 李亚康[1] 任伟光[1] 黄林芳[1] 

机构地区：[1]中国医学科学院、北京协和医学院药用植物研究所,北京100193

出　　处：《药学学报》2014年第12期1730-1738,共9页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目(81274013,81473315);国家自然科学基金重点项目(81130069)

摘　　要：本文通过实验,并结合Gen Bank数据库序列,分析ITS、ITS2、psb A-trn H、mat K、rbc L 5条常用条形码共978条序列在种内与种间变异、barcoding gap、系统发育树等方面对百合属植物的鉴别能力,并采用最近距离法(nearest distance)和相似性搜索算法(BLAST1)评价5条序列的鉴定成功率。研究结果显示,不同条形码序列可以较好的鉴别百合属内不同物种。ITS序列鉴定成功率较高,种内种间差异显著,系统发育树可以很好地将不同物种分开。结果表明DNA条形码技术可以准确地鉴别百合属植物,ITS序列可以作为鉴别百合属不同物种的优选序列。To establish a new method for identifying genus of Lilium by DNA barcoding technology, ITS, ITS2, psbA-trnH, matK and rbcL sequences were analyzed in term of variation of inter- and intra-species, barcoding gap, neighbor-joining tree to distinguish genus of Lilium based on 978 sequences from experimental and GenBank database, and identification efficiency was evaluated by Nearest distance and BLAST1 methods. The results showed that DNA barcoding could identify different species in genus of Liliurn. ITS sequence performed higher identification efficiency, and had significant difference between intra- and inter-species. And NJ tree could also divide species into different clades. Results indicate that DNA barcoding can identify genus of Lilium accurately. ITS sequence can be the optimal barcode to identify species of Lilium.

关 键 词：百合属 DNA条形码 分子鉴别 ITS 

分 类 号：R931[医药卫生—生药学]