脊髓NK1受体与非NMDA受体对大鼠心脏-躯体运动反射的调节作用  

Modulatory effect of neurokinin-1 and non-N-methyl-D-aspartate receptors on cardiosomatic reflex in rat spinal cord

作　　者：韩曼[1] 刘晓华[1] 杜剑青[2] 

机构地区：[1]陕西中医学院生理学教研室,陕西咸阳712046 [2]西安交通大学医学院生理学与病理生理学系,陕西西安710061

出　　处：《南方医科大学学报》2014年第12期1728-1732,共5页Journal of Southern Medical University

基　　金：国家自然科学基金(31171068);陕西中医学院博士基金(504010223)~~

摘　　要：目的观察脊髓水平神经激肽-1(NK1)受体和非N-甲基-D-天门冬氨酸(NMDA)受体对大鼠心脏-躯体运动反射的调节作用。方法鞘内注射NK1受体激动剂Sar-SP及拮抗剂CP-96345与非NMDA受体激动剂NMDA及拮抗剂6,7-二硝基喹喔啉-2,3-二酮(DNQX),观察心包内注射缓激肽(BK)诱发大鼠心脏-躯体运动反射的变化,该反射以背斜方肌肌电(EMG)为观测指标。结果鞘内注射NK1受体激动剂Sar-SP后,对心包内BK诱发的背斜方肌EMG有明显的易化作用(P<0.05),这种易化作用被鞘内预先注射NK1拮抗剂CP-96345完全阻断;鞘内注射非NMDA受体激动剂NMDA后,对心包内BK诱发的背斜方肌EMG有显著的易化作用(P<0.05),这种易化作用被鞘内预先注射非NMDA受体拮抗剂DNQX部分阻断;鞘内联合注射Sar-SP与NMDA,对EMG的易化作用较单一注射Sar-SP或NMDA显著增强(P<0.05)。结论脊髓水平的NK1受体与非NMDA受体参与了大鼠心脏-躯体运动反射的调节。Objective To investigate the role of neurokinin-1 （NK1） receptor and non-N-methyl-D-aspartate （non-NMDA） receptor in modulating the cardiosomatic reflex in the spinal cord of rat. Methods The effects of intrathecal injection of NK1 receptor agonist Sar-SP and antagonist CP-96345 and non-NMDA receptor agonist NMDA and antagonist 6, 7-dinitro-1,4-dihydro-quinoxaline-2,3-dione （DNQX） on the cardiosomatic reflex were observed in rats. The changes of the cardiosomatic reflex were monitored by observing the electromyogram （EMG） responses of the dorsal spinotrapezius muscle to intrapericardial bradykinin （BK） injection. Results Intrathecal injection of Sar-SP significantly facilitated EMG responses to intrapericardial BK injection （P〈0.05）, which was completely blocked by intrathecal injection of NK1 antagonist CP-96345. Similarly, intrathecal injection of NMDA obviously facilitated the EMG responses （P〈0.05）, which was partially reversed by intrathecal injection of non-NMDA receptor antagonist DNQX. Intrathecal injection of Sar-SP along with NMDA significantly increased EMG as compared with single administration of Sar-SP or NMDA （P〈0.05）. Conclusion The spinal cord levels of NK1 receptor and non-NMDA receptor are involved in the modulation of the cardiosomatic reflex in rats.

关 键 词：脊髓 心脏-躯体运动反射 肌电 神经激肽-1受体 非-N-甲基-D-天门冬氨酸受体 

分 类 号：R541[医药卫生—心血管疾病]