长脉冲超声条件下脂质体微泡协同rt-PA溶解微血栓  

Lipid microbubble synergistic rt-PA thrombolysis under long-pulsed ultrasound enhanced condition

作　　者：陈向辉[1] Seunghan Ha 崔凯[3] 查道刚[3] 宾建平[3] 李自成[1] 刘伊丽[3] 

机构地区：[1]暨南大学附属第一医院心内科,广东广州510630 [2]韩国忠北卫生科学大学居勤学院 [3]南方医科大学附属南方医院心内科,广东广州510515

出　　处：《中国医学影像技术》2014年第12期1769-1772,共4页Chinese Journal of Medical Imaging Technology

基　　金：广东省自然科学基金(S2013040015434);高等学校博士学科点专项科研基金(20134401120005);暨南大学第一临床医学院科研培育专项基金(2013202)

摘　　要：目的观察长脉冲超声（US）条件下脂质体微泡造影剂协同重组组织型纤溶酶原激活剂（rt-PA）溶解微血栓的疗效。方法采集6名健康受试者全血制备200～300μm的微血栓,根据不同干预方式分为单纯rt-PA组、rt-PA＋低占空比US组（5000个循环）、rt-PA＋高占空比US组（600 000个循环）,另将仅US（600 000个循环）照射但无声学微泡协同作为单纯US组。根据辐照系统内压力减低程度评价溶栓的疗效。结果单纯rt-PA组溶栓率与rt-PA＋低占空比US组比较,差异无统计学意义（P〉0.05）,rt-PA＋高占空比US组溶栓率高于单纯rt-PA组和rt-PA＋低占空比US组（P均〈0.01）。结论长脉冲US声学微泡辅助rt-PA溶栓效果良好,但此协同作用需在低声压条件下依赖一定比例的占空比才能实现。Objective To observe the synergistic effect of lipid microbubble synergistic recombinant tissue plasminogen activator（rt-PA）thrombolysis under long-pulsed ultrasound（US）enhanced condition.Methods The whole blood of six healthy subjects were collected and 200—300μm thrombi was prepared.All blood samples were divided into rt-PA group,rt-PA＋low duty cycle group（5000cycle）,rt-PA＋high duty cycle group（600 000cycle）according to different intervene methods,meanwhile ultrasound irradiate without lipid microbubble as US group.Intra-system pressure was monitored to evaluate of the efficacy of thrombolysis.Results There was no significantly differences in thrombolytic rate between rt-PA＋low duty cycle group and rt-PA group（P〉0.05）.The thrombolytic rate in rt-PA＋high duty cycle group was higher than that of rt-PA group and rt-PA＋low duty cycle group（both P〈0.01）.Conclusion rt-PA thrombolys assisted with long-pulsed US acoustic microbubbles is good,which is dependent on duty-cycle under low acoustic pressure condition.

关 键 词：超声检查 微泡 纤溶酶原激活剂 溶栓治疗 

分 类 号：R3[医药卫生—基础医学] R445.1