3.0T BOLD-fMRI评价丹参注射液对大鼠对比剂肾病的保护作用:与病理对照研究  

Protective effect of salvia miltiorrhiza for contrast-induced nephropathy in rats assessed by 3.0T blood oxygen level-dependent functional MRI:Correlated with pathology

作　　者：冉鹏程[1] 刘玉品[1] 刘波[1] 杨海峰[2] 陈志远[1] 王春华[1] 

机构地区：[1]广州中医药大学第二临床医学院放射科,广东广州510120 [2]广州中医药大学第二临床医学院病理科,广东广州510120

出　　处：《中国医学影像技术》2014年第12期1788-1791,共4页Chinese Journal of Medical Imaging Technology

摘　　要：目的采用血氧水平依赖功能MRI(BOLD-fMRI)技术评估丹参注射液对大鼠对比剂肾病(CIN)的保护作用。方法将60只SD大鼠随机均分为丹参组和对照组进行BOLD-fMRI,分别于注射对比剂前、后连续注射丹参注射液及生理盐水7天(每天1次);于注射对比剂前、注射后20min、24h、48h、72h和7天点每组各取5只大鼠行BOLD-fMRI扫描,测量大鼠肾脏外髓部R*2值,后取大鼠肾脏行病理检查,评价外髓部肾小管损伤情况,分析和比较两组R*2值及病理损伤变化过程和差异,评价R*2值与病理损伤相关性。结果两组大鼠外髓部R*2值于注射对比剂后20min均增高,随后对照组先回落后复升高,丹参组持续回落;注射对比剂前及注射后20min和48h丹参组R*2值高于对照组(P均<0.05),注射后72h和7天丹参组R*2值低于对照组(P均<0.05)。病理结果表明两组大鼠注射对比剂后20min外髓部肾小管均出现损伤,注射后7天对照组病理损伤评分达最高,丹参组恢复至注射前水平;注射对比剂后20min丹参组病理损伤评分明显高于对照组(P<0.01),24h^7天丹参组病理损伤评分均低于对照组,除24h外,余时间点两组间差异均有统计学意义(P均<0.05)。肾脏外髓部R*2值与肾小管损伤病理损伤评分呈正相关(右肾:r=0.58,左肾:r=0.52,左右肾合计:r=0.56;P均<0.001)。结论丹参注射液对CIN具有预防和治疗作用,其效果可通过3.0TBOLD-fMRI进行检测。Objective To explore the protective effect of salvia miltiorrhiza injection（SMI）for contrast-induced nephropathy（CIN）in rats using 3.0Tblood oxygen level-dependent functional MRI（BOLD-fMRI）.Methods Totally 60 rats were randomly divided into SMI group and control group（each n=30）and underwent BOLD-fMRI.The SMI and saline was administrated one time everyday before and 1—6days after injection of contrast agent（CA）in the two groups,respectively.BOLD-fMRI was performed on 5rats of each group before and 20 min,24h,48 h,72hand 7days after injection of contrast agent to measure the R2^＊ value of kidney outer medulla and then the degree of renal tubule injury in the outer medulla was assessed by pathology.The change processes of R2^＊ value and renal tubule injury were analyzed and compared between different groups and time points.The correlation of R2^＊ value and renal tubule injury was analyzed.Results R2＊ value of both groups increased significantly 20 min after injection of CA,then falled-rised in control group and fall continiously in SMI group.R2＊value in SMI group was higher before and 20 min,48hafter injection of CA,and lower 72hand7 days after injection of CA compared with control group（all P〈0.05）.Pathology results showed that renal tubule injury occurred 20 min after injection of CA,which reached to the most serious degree in control group and recovered to the preinjective level in SMI group 7days after injection of CA.The pathological injury score of SMI group was significantly higher 20 min after injection of CA and lower 24 hto 7days after injection of CA compared with control group（all P〈0.05,except for 24h）.R2^＊ had positive correlation with the pathological injury score of renal tubule（right renal：r=0.58,left renal：r=0.52,two renals：r=0.56,all P〈0.001）.Conclusion SMI plays an important role in prevention and protection of CIN,which can be detected by 3.0TBOLD-fMRI.

关 键 词：对比剂肾病 磁共振成像 丹参注射液 

分 类 号：R445.2[医药卫生—影像医学与核医学] R-332[医药卫生—诊断学]