岩大戟内酯B对耐阿霉素白血病细胞株HL-60/ADR细胞凋亡的影响及其机制  

Effect of Jolkinolide B on apoptosis of adriamycin-resistant leukemia cell lines HL-60/ADR and its mechanism

作　　者：白雪[1] 赵鹤[2] 何平[1] 

机构地区：[1]中国医科大学附属盛京医院老年病科,辽宁沈阳110004 [2]中国医科大学附属盛京医院耳鼻喉科,辽宁沈阳110004

出　　处：《新乡医学院学报》2014年第12期990-993,共4页Journal of Xinxiang Medical University

摘　　要：目的探讨死亡受体通路在岩大戟内酯B诱导的耐阿霉素白血病细胞株HL-60/ADR细胞凋亡中的作用。方法采用Annexin V异硫氰酸荧光素/碘化丙啶(FITC/PI)双染流式细胞术检测HL-60/ADR细胞凋亡;利用Western blot检测Fas/Fas L蛋白的表达;采用荧光比色法测定细胞内caspase-8和caspase-3蛋白酶活性。结果 HL-60/ADR细胞的凋亡率随着岩大戟内酯B浓度的增加及作用时间的延长而逐渐增加。当岩大戟内酯B浓度为40 mg·L-1时,作用24、48及72 h后早期凋亡率和总凋亡率与0 h比较,差异均有统计学意义(P<0.05或P<0.001);当浓度为20、40、80 mg·L-1时,作用48 h后早期凋亡率和总凋亡率与0 mg·L-1比较,差异均有统计学意义(P<0.05或P<0.001)。Fas/Fas L蛋白的表达随着岩大戟内酯B作用时间的延长逐渐增加;caspase-8和caspase-3蛋白酶的活性亦逐渐增加。作用24、48及72 h后caspase-3和caspase-8蛋白酶活性与0 h比较,差异均有统计学意义(P<0.05或P<0.001)。结论岩大戟内酯B能够诱导耐阿霉素白血病细胞株HL-60/ADR细胞凋亡,Fas/Fas L通路参与了岩大戟内酯B诱导的HL-60/ADR细胞凋亡过程。Objective To explore the role of Fas/FasL pathway in the apoptosis of adriamycin-resistant leukemia cell lines HL-60/ADR induced by Jolkinolide B. Methods The apoptosis of HL-60/ADR cell was detected by Annexin V fulorescein isothiocyanate/propidiumiodide（FITC/PI） staining. The expressions of Fas/FasL were detected by Western blot. The activity of caspase-8 and caspase-3 was detected by fluorometry. Results The HL-60/ADR apoptosis rate increased with the increase of concentration of Jolkinolide B and extension of action time of Jolkinolide B. The early apoptosis rates and total apoptosis rates induced by 40 mg·L-1Jolkinolide B were significantly higher at 24,48 and 72 h than those at 0 h （P 〈 0.05, P 〈 0. 001 ）. The early apoptosis rates and total apoptosis rates induced by 20,40 and 80 mg ·L-1 Jolkinolide B were significantly higher than those of 0 mg·L-1 at 48 h （ P 〈 0.05, P 〈 0.001 ）. The expression of Fas/FasL protein and activities of caspase-3 and caspase-8 increased with the extension of action time of Jolkinolide B. The activities of caspase-3 and caspase-8 were significantly higher at 24,48 and 72 h than those at 0 h （P 〈 0.05,P 〈 0.001 ）. Conclusion Jolkinolide B can induce the apoptosis of adriamycin-resistant leukemia cell lines HL-60/ADR. Fas/FasL way participates in the apoptosis of HL-60/ADR ceils induced by Jolkinolide B.

关 键 词：岩大戟内酯B HL-60/ADR细胞 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]