肿瘤坏死因子-α和白细胞介素-1β在慢性乙型肝炎和原发性肝癌患者血清及肝组织中的表达  

Expression of tumor necrosis factor-α and interleukin-1β in the serum and hepatic tissues of patients with chronic hepatitis B or primary hepatic cancer

作　　者：向保云 李红德[1] 徐江海[1] 申林杰 徐嘉[1] 王雷[2] 

机构地区：[1]安阳市第五人民医院肝病科 [2]新乡医学院第一附属医院普外科

出　　处：《新乡医学院学报》2014年第12期999-1001,共3页Journal of Xinxiang Medical University

摘　　要：目的探讨肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)在慢性乙型肝炎(CHB)和原发性肝癌(PHC)患者血清及肝组织中的表达水平。方法应用酶联免疫吸附测定法检测45例CHB患者(CHB组)、45例PHC患者(PHC组)及45例健康人(对照组)血清中TNF-α、IL-1β水平,采用免疫组织化学方法检测CHB和PHC患者肝组织中TNF-α、IL-1β蛋白的表达,并对3组结果进行比较。结果 CHB组患者血清TNF-α和IL-1β水平高于对照组(P=0.000),PHC组患者血清TNF-α和IL-1β水平显著高于CHB组(P=0.000)。PHC组织中TNF-α和IL-1β蛋白阳性表达显著高于CHB组织(P=0.000)。结论 TNF-α和IL-1β是重要的促炎性细胞因子,在CHB和PHC患者的肝损伤过程中发挥重要作用,可作为临床生物治疗的靶点。Objective To study the expression of tumor necrosis factor-α（TNF-α）and interleukin-1β（IL-1β） in the serum and hepatic tissues of patients with chronic hepatitis B （CHB） or primary hepatic cancer（PHC）. Methods The levels of serum TNF-α and IL-1β in 45 patients with CHB（ CHB group） ,45 patients with PHC（ PHC group） and 45 healthy people （control group） were detected by enzyme-linked immunosorbent assay. The expression of TNF-α and IL-1β in CHB and PHC tissues were detected by immunohistochemistry method. The results were compared among the three groups. Results The levels of serum TNF-α and IL-1β in CHB group were significantly higher than those in control group（P = 0.000）. The levels of serum TNF-α and IL-1β in PHC group were significantly higher than those in CHB group （P = 0. 000）. The positive expression of TNF-α and IL-1β in PHC tissues was significantly higher than that in CHB tissues （P = 0. 000）. Conclusion TNF-α and IL-1 β as the important proinflammatory cytokines ,play an important role in the process of liver injury in patients with CHB or PHC, and can be used as clinical biological treatment targets.

关 键 词：肿瘤坏死因子-Α 白细胞介素-1Β 慢性乙型肝炎 原发性肝癌 

分 类 号：R512.6[医药卫生—内科学]