8-OH-DPAT、达泊西汀和甲基安非他明对雄性大鼠性行为的影响  

Effects of 8-OH-DPAT, dapoxetine and methamphetamine on sexual behaviors of male rats

作　　者：覃喜军[1] 涂冬萍[1] 黄杰[2,4] 马小军[1,3] 韦荣昌[1,2] 莫长明[2] 潘丽梅[2] 

机构地区：[1]中国医学科学院,北京协和医学院药用植物研究所,北京100193 [2]中国医学科学院药用植物研究所广西分所 [3]中国医学科学院药用植物研究所云南分所 [4]广西中医药大学

出　　处：《中国男科学杂志》2014年第9期8-12,共5页Chinese Journal of Andrology

摘　　要：目的：探讨3种试剂8-OH-DPAT、达泊西汀和甲基安非他明对雄鼠性行为的影响。方法将120只具有射精经历的雄鼠随机分为4组，每组30只，分别为对照组、8-OH-DPAT、达泊西汀和甲基安非他明给药组。分别给予雄鼠注射生理盐水、8-OH-DPAT（0.5mg/kg、1.0mg/kg、2.0mg/kg 腹腔注射）、达泊西汀（30mg/kg、45mg/kg、60mg/kg 腹腔注射）、甲基安非他明（0.5mg/kg、1.0mg/kg、2.0mg/kg 腹腔注射）一次，注射时间分别为实验前20min、1h、15min。然后与发情良好的雌鼠1：1合笼，用摄像机录像观察并记录他们发生性行为特征指标。各组实验重复3次。结果与对照组相比，0.5mg/kg、1.0mg/kg 8-OH-DPAT有显著性差异（P＜0.05），减小骑跨、插入、射精潜伏期及射精后间隔期，增加射精次数和提高交配效率，进而促进雄鼠性行为。达泊西汀各剂量组均对雄鼠射精有显著性的抑制作用（P＜0.05），延长射精潜伏期及射精后间隔期，减少射精次数及降低交配效率。0.5mg/kg安非他明对雄鼠性行为有显著性的促进作用（P ＜0.05），减小骑跨、插入潜伏期和射精后间隔期，增加射精次数，但1.0mg/kg安非他明延缓雄鼠射精，而到2.0mg/kg时几乎完全抑制雄鼠性行为。结论5-HT1A受体激动剂8-OH-DPAT促进雄鼠射精。选择性5-HT再摄取抑制剂（SSRI）达泊西汀延迟射精的发生，可用于早泄的治疗。精神兴奋剂甲基安非他明对性行为的影响与剂量有关。Objective To investigate the effects of 8-OH-DPAT, Dapoxetine and Methamphetamine on sexual behavior of male rats. Methods One hundred and twenty adult male rats who have experienced ejaculation were divided into four groups includingthe control（30 rats）, 8-OH-DPAT group（30 rats）, Dapoxetine group（30 rats） and Methamphetamine groups（30 rats）, rats in which group respectively received one time injection of 0.9% sodium chloride, 8-OH-DPAT （0.Smg/ kg, 1.0mg/kg, 2.0mg/kg S.C.）, Dapoxetine （30mg/kg, 45mg/kg, 60mg/kg S.C.）, Methamphetamine （0.Smg/kg, 1.0mg/kg, 2.0mg/kg）, and the injection time points were set at 20rain, lh, 15min before experiment. Then male rats were lived with good estrus female mate, sex characteristic index was recorded by a video camera. Each group experiment was repeated three times. Results Compared with control group, 0.5mg/kg and 1.0mg/kg 8-OH-DPAT by intravenous injection both existed in significant difference （P〈0.05）. They produced facilitation of the male rat sexual behavior, characterized by a decrease in mount, intromission, ejaculation latency and post-ejaculatory interval, by increase in ejaculation frequency and improvement in copulation efficiency; All doses of Dapoxetine had significant inhibition to ejaculation in male rats （P〈0.05）, as evidenced by extension in ejaculation latency and post-ejaculatory interval, reduction in ejaculation frequency and copulation efficiency; Methamphetamine （0.5mg/kg S.C.） promoted remarkably sexual behavior in male rats （P〈0.05）, as observed by decrease in mount, intromission, ejaculation latency and post-ejaculatory interval, increase in mount and ejaculation frequency. However, 1.0mg/kg Methamphetamine delayed ejaculation and 2.0mg/kg Methamphetamine almost completely inhibited sexual behavior in male rats. Conclusion The agonist of5-HT1A 8-OH-DPAT facilitates and serotonin reuptake inhibitor （SSRI） dopoxetine delays ejaculation in male rat, which the latter can be used for treatment in prematu

关 键 词：8-OH—DPAT 达泊西汀 甲基安非他明 大鼠 性行为 动物 

分 类 号：R338[医药卫生—人体生理学]