益母草总黄酮对去卵巢大鼠下丘脑和海马雌激素受体α及β mRNA表达的影响  

Effect of Total Flavonoids of Herba Leonuri on the mRNA Expression of Estrogen Receptor α and β in Hypothalamus and Hippocampus in Ovariectomized Rats

作　　者：宋静荣[1] 

机构地区：[1]北华大学附属医院,吉林吉林132011

出　　处：《北华大学学报（自然科学版）》2014年第6期747-749,共3页Journal of Beihua University（Natural Science）

基　　金：吉林省卫生厅科技发展计划项目(20122010)

摘　　要：目的：观察益母草总黄酮对去卵巢大鼠下丘脑和海马雌激素受体α及β mRNA表达的影响，探讨其对绝经后骨质疏松的作用机制。方法选取10~11月龄SD雌性大鼠48只，随机分为对照组、去势组、益母草总黄酮组和雌二醇组，以双侧卵巢切除法建立大鼠骨质疏松模型。益母草总黄酮组、雌二醇组分别用益母草总黄酮、雌二醇给药4个月，采用RT-PCR法检测各组下丘脑和海马雌激素受体α及β mRNA的表达。结果大鼠卵巢切除后，血清中雌二醇水平、椎骨骨密度、子宫湿重、下丘脑和海马雌激素受体α及βmRNA的表达均明显下降。雌二醇治疗后血清中雌二醇水平、椎骨骨密度、子宫湿重升高，下丘脑和海马β mRNA的表达均明显下降。益母草总黄酮治疗后，血清中雌二醇水平、椎骨骨密度、下丘脑和海马雌激素受体α及β mRNA的表达均明显升高，子宫湿重无明显改变。结论益母草总黄酮对切除卵巢所致的骨质疏松大鼠有防治作用，对子宫无不良影响。Objective To explore the effect of total flavonoids of Herba leonuri（ TFH） on the mRNA expressions of estrogen receptor alpha （ ERα） and estrogen receptor beta （ ERβ） in hippocampus and hypothalamus in ovariectomized （ OVX ） Sprague-Dawley （ SD ） rats, and the mechanism of TFH against postmenopausal osteoporosis. Method Forty-eight female SD rats（10~11 months） were randomly divided into 4 groups：a sham group,an ovariectomy group,a TFH group,and an estradiol group. The RT-PCR was performed to determine the mRNA expression of ERα and ERβ in hypothalamus and hippocampus. Results After the ovariectomy, the serum estradiol level,bone mineral density（ BMD） of vertebra,wet weight of uterus,and the mRNA expressions of ERα and ERβ in hypothalamus and hippocampus were markedly decreased in OVX rats;after the treatment with TFH,the serum estradiol level,bone mineral density（BMD） of vertebra,wet weight of uterus,and the mRNA expressions of ERα and ERβ in hypothalamus and hippocampus were increased except that the wet weight of uterus did not change. Conclusion TFH can improve BMD of vertebra in the OVX rats without untoward effects on the uterus.

关 键 词：总黄酮 卵巢切除术 雌激素受体Α 雌激素受体Β 

分 类 号：R285.5[医药卫生—中药学]