早发冠心病患者血小板CD_(40)配体表达变化及意义  

Variation and effect of the expression of platelet CD_(40) ligand in patients with premature coronary heart disease

作　　者：李彬[1] 卢成志[2] 夏大胜[2] 赵剑[1] 张峰[2] 李超[2] 

机构地区：[1]天津医科大学一中心临床学院,天津300192 [2]天津市第一中心医院

出　　处：《山东医药》2014年第43期16-18,共3页Shandong Medical Journal

基　　金：天津市卫生局科技基金资助项目(2010KY06)

摘　　要：目的观察早发冠心病患者的血小板CD40配体(CD40L)表达变化并探讨其意义。方法因胸闷、胸痛而接受冠状动脉造影的患者200例,造影显示冠状动脉狭窄140例(早发冠心病组)、冠状动脉无狭窄60例(对照组)。采用流式细胞技术测定血小板CD40L平均荧光强度,ELISA法检测血浆组织因子(TF)及组织因子途径抑制物(TFPI)。结果早发冠心病组血小板CD40L荧光强度为(19.3±6.1)a.u,血浆TF为(70.0±9.5)ng/L、TFPI为(68.0±9.4)μg/L、TF/TFPI为1.3±0.1,对照组分别为(8.8±2.4)a.u、(55.3±7.5)ng/L、(59.3±7.7)μg/L、0.9±0.1,两组相比,P<0.05或P<0.01;血小板CD40L荧光强度与早发冠心病患者血浆TF、TFPI、TF/TFPI均呈正相关(r分别为0.43、0.32、0.30,P均<0.01);血小板CD40L荧光强度与早发冠心病患者血浆TF水平呈独立正相关(B=0.4,P<0.01)、与早发冠心病发病呈独立正相关(B=0.8,P<0.01)。结论早发冠心病患者血小板CD40L表达增强,高CD40L水平可能通过促进TF的表达而促进早发冠心病的发生发展。Objective To explore variation and effect of the expression of CD 40 ligand（CD40L） on platelets in prema-ture coronary heart disease patients .Methods Selected 200 patients experienced coronary artery angiography due to chest congestion or pain as experimental subjects , which were divided as premature coronary heart disease group （ 140 cases of coronary artery stenosis shown on imaging ） and control group （ 60 cases without stenosis ） .Flow cytometry was used for measurement of mean fluorescence intensity about CD 40 L on platelets.Tissue factor （TF） and tissue factor pathway inhibi-tor （ TFPI） were detected by method of ELISA .Results In premature coronary heart disease group , the data of fluores-cence intensity[（19.3 ±6.1） a.u],TF in plasma[（70.0 ±9.5） ng/L],TFPI[（68.0 ±9.4）μg/L], TF/TFPI（1.3 ± 0.1） were collected.Furthermore, the figures were detected as（8.8 ±2.4） a.u,（55.3 ±7.5）ng/L,（59.3 ±7.7）μg/L, （0.9 ±0.1 ） in control group correspondingly .There was significant difference between two groups （ P〈0.05 or P 〈0.01）.It had revealed positive correlation between fluorescence intensity of CD 40L on platelets and TF in plasma, TFPI, TF/TFPI of premature coronary heart disease patients （r were 0.43, 0.32, 0.30;all P〈0.01）.In addition, fluorescence intensity of CD40L on platelets was independenty positive correlated with TF level in plasma （B=0.4, P〈0.01） and onset （B=0.8, P〈0.01） of premature coronary heart disease.Conclusions The expression of platelet CD40L shows an in-creasing trend in premature coronary heart disease patients .Besides, high level of CD40 L may accelerate the occurrence and development of premature coronary heart disease by stimulating the expression of TF .

关 键 词：冠状动脉性心脏病 血小板CD40配体 组织因子 组织因子途径抑制物 

分 类 号：R541.4[医药卫生—心血管疾病]