绿豆(Vigna Radiata)代谢物组成的核磁共振定量分析  

Quantitative Analysis of Metabolites in Mungbean(Vigna Radiata) Extracts using NMR Techniques

作　　者：吴香玉[1,2] 李宁[1,2] 唐惠儒[1] 

机构地区：[1]中国科学院生物磁共振分析重点实验室、波谱与原子分子物理国家重点实验室、武汉磁共振中心、中国科学院武汉物理与数学研究所,湖北武汉430071 [2]中国科学院大学,北京100049

出　　处：《波谱学杂志》2014年第4期548-563,共16页Chinese Journal of Magnetic Resonance

基　　金：国家自然科学基金资助项目(21175149,21221064)

摘　　要：绿豆(Vigna radiata)是重要的经济作物之一,具有抗氧化、抗炎和抗菌作用.然而迄今为止,绿豆代谢物组成信息仍不完善.为了系统地定量研究绿豆代谢物组成,该研究利用DOSY,J-RES,COSY,TOCSY,HSQC,HMBC和1D 1H NMR谱图在鄂绿4号(ElüNo.4)绿豆水相提取物中鉴定和归属了48种代谢物,确定了绿豆中棉籽糖家族寡糖的构成,并且利用全弛豫谱对其中44种代谢物进行了定量分析.Mungbean （Vigna radiata） is an important crop that has various beneficial functions such as antioxidant and anti-inflammatory activities. However, the metabolites in mungbean have only been partially identified and quantified in previous studies. In this study, we systematically identified and assigned 48 metabolites in mungbean （Elü No.4） seeds with J-RES, COSY, TOCSY, HSQC, HMBC and 2D NMR spectra. In particular, the composition of raffinose family oligosaccharides in the extracts was determined using DOSY experiments. Most of the metabolites in the extracts were quantified. This study will provide useful information for mungbean metabolomic and nutritional studies.

关 键 词：核磁共振(NMR) 归属 2DNMR 绿豆 定量 

分 类 号：O482.53[理学—固体物理]