不同脂肪酸种类及浓度对棉纤维分化发育的影响  

Effects of different kinds and concentrations of fatty acids on differentiation and development of cotton fiber

作　　者：孙英超[1] 刘伟[1] 何秋伶[1] 祝水金[1] 陈进红[1] 

机构地区：[1]浙江大学农业与生物技术学院农学系/浙江省作物种质资源重点实验室,杭州310058

出　　处：《浙江大学学报（农业与生命科学版）》2014年第6期621-626,共6页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：国家自然科学基金资助项目(30971820);国家科技重大专项"转基因生物新品种培育"(2013ZX08005-005)

摘　　要：以陆地棉标准系TM-1为材料,利用棉花胚珠离体培养技术并结合对纤维分化发育状态的扫描电镜观察,研究豆蔻酸(C14)、棕榈酸(C16)、硬脂酸(C18)、油酸(C18:1)、亚油酸(C18:2)、花生酸(C20)、山嵛酸(C22)、木蜡酸(C24)等不同链长脂肪酸以及不同浓度棕榈酸对棉纤维分化发育的影响.结果表明:在离体培养基中添加棕榈酸(C16)对棉纤维的分化发育及伸长有较明显的促进作用,特别是在培养基中添加5.0μmol/L棕榈酸纤维的伸长最明显,其纤维平均长度为1.086mm,比对照增加53.4%,差异具统计学意义(P<0.01),而棕榈酸浓度过高则抑制纤维的伸长;木蜡酸(C24)对纤维伸长也表现出一定的促进作用,纤维平均长度比对照增加21.9%(P<0.05);扫描电镜观察表明,在5.0μmol/L棕榈酸处理下胚珠表面光滑且突起多、密,分布均匀;添加硬脂酸、油酸或山嵛酸的处理几乎没有出现突起.说明在本试验条件下,在棉花胚珠离体培养中促进纤维分化发育的棕榈酸适宜浓度为5.0μmol/L.Summary As a kind of seed fiber , cotton fiber was differentiated by epidermal cells of the seed , and was adhered to seed surface . Cotton kernel is rich in fat , protein and other ingredients . However ,whether these ingredients affect the differentiation and development of cotton fiber , and subsequently influence the yield and quality has become a hot topic in the related research area recently . It also has a pivotal role in the regulation to cotton production and quality formation . For exploring the function of fatty acid in the developing process of cotton fiber , this study took upland cotton standard system TM‐1 as experimental material , to investigate the effect of different kinds of fatty acids on fiber differentiation and development along with fiber length . The experiment was conducted by adding 5 .0 μmol/L different kinds of fatty acids respectively （ including nutmeg acid , palmitic acid , stearic acid , oleic acid , linoleic acid , peanut acid , docosanoic acid and wood wax acid ,） in the in vitro ovule culture medium . These fatty acids were with varied chain length . The medium without any fatty acid was involved as a control . Scanning electron microscope （ SEM ） was used to observe the differential and developmental situation of ovule surface after 2 d culture , and the fiber length was measured after 2 weeks . Thereafter , the palmitic acid was selected to study the effect of concentration gradient on fiber development ; in other words , different palmitic acid concentrations of 0 .01 , 0 .1 , 1 .0 , 5 .0 , 10 .0 , 20 .0 , 25 .0 , 30 .0 μmol/L were added into medium to investigate their effects on the differentiation and development of cotton fiber by using SEM and length measurement .The results showed that in the medium with 5 .0 μmol/L palmitic acid , ovule epidermal ridges were much more dense and well‐distributed than in the control and the other treatments . Almost no ridge was observed in the treatments of stearic acid , oleic acid and docosanoic acid . The fiber leng

关 键 词：陆地棉 胚珠离体培养 脂肪酸 棕榈酸 纤维分化发育 

分 类 号：S562[农业科学—作物学] Q24[生物学—细胞生物学]