检测水貂出血性肺炎绿脓杆菌的比色LAMP法的建立与应用  

Rapid detection of Pseudomonas aeruginosa in mink hemorrhagic pneumonia by colorimetric LAMP assay

作　　者：韩明明[1] 刘旭平[1] 母连志[1,2] 赵静[1] 刘慧[1,2] 刘晓飞[1] 

机构地区：[1]吉林大学动物医学学院,吉林长春130062 [2]吉林省五星动物保健药厂,吉林长春130062

出　　处：《中国兽医学报》2014年第4期583-587,共5页Chinese Journal of Veterinary Science

基　　金：吉林省五星动物保健药厂研发基金(2013003)

摘　　要：为了快速有效检测水貂出血性肺炎病原绿脓杆菌,本研究结合金属指示剂HNB特性建立了快速检测绿脓杆菌比色LAMP法。根据绿脓杆菌外毒素A基因设计引物,建立了检测LAMP法;并且对该方法进行了特异性、灵敏性分析;同时进行了初步应用。结果显示,该方法特异性强,灵敏性好,可检测167CFU/mL的菌体,对病貂肺脏检出率高。结果表明,该比色LAMP法可以有效地检测水貂出血性肺炎绿脓杆菌。In order to detect Pseudomonas aeruginosa of mink hemorrhagic pneumonia quickly and effectively,colorimetric LAMP assay based on indicator HNB was established for rapid detection of Pseudomonas aeruginosa. Pseudornonas exotoxin A gene primers were designed to establish colorimetric LAMP assay, and the specificity and sensitivity were measured. The results showed that the assay was specific and sensitive, and it could detect the 167 CFU/mL bacteria. Pseudo- monas aeruginosa from lung of diseased mink could also been detected. The colorimetric LAMP assay could be used to detect Pseudomonas aeruginosa effectively in hemorrhagic pneumonia mink.

关 键 词：水貂出血性肺炎 绿脓杆菌 外毒素A基因 比色LAMP法 

分 类 号：S852.61[农业科学—基础兽医学]