PYY对LPS诱导小鼠腹腔巨噬细胞TNF-α、IL-6分泌的影响  

Effects of peptide YY on secretion of TNF-α and IL-6 in LPS-induced mouse peritoneal macrophage

作　　者：李志强[1] 崔敏[1] 陈福军 李苏楠[1] 张晓静[1] 薛文静[1] 刘红梅[1] 陈巍[1] 柳巨雄[1] 

机构地区：[1]吉林大学动物医学学院,吉林长春130062 [2]长春五棵树经济开发区动物卫生监督所,吉林长春130401

出　　处：《中国兽医学报》2014年第4期588-592,共5页Chinese Journal of Veterinary Science

基　　金：吉林大学种子基金资助项目(450060481363);吉林大学农学部青年科研基金资助项目(4305050102P4);吉林大学<大学生创新创业训练计划资助>项目(2012C81365)

摘　　要：为明确PYY对巨噬细胞炎性细胞因子分泌的调节作用,本试验分离培养健康小鼠腹腔巨噬细胞,不同浓度PYY预处理后,以LPS刺激。ELISA方法检测细胞培养上清中TNF-α、IL-6含量,半定量PCR方法检测细胞中TNF-α、IL-6mRNA表达变化。结果显示：高浓度的PYY1-36（10-9-10-7 mol/L）和PYY3-36（10-8-10-7 mol/L）对LPS诱导小鼠腹腔巨噬细胞TNF-α分泌具有显著抑制作用（P〈0.05）;PYY1-36对LPS诱导小鼠腹腔巨噬细胞IL-6分泌无明显作用（P〉0.05）;不同浓度PYY3-36（10-11-10-7 mol/L）对LPS诱导小鼠腹腔巨噬细胞IL-6分泌均具有显著抑制作用（P〈0.05）。表明PYY对LPS诱导小鼠腹腔巨噬细胞炎性细胞因子TNF-α及IL-6的分泌具有一定的抑制作用,提示PYY可能通过抑制炎性细胞因子的分泌而抑制炎症性疾病的发生发展。To investigate the effects of peptide YY（PYY）on secretion of pro-inflammatory cyto- kines in maerophage,isolated mouse peritoneal macrophages that had been pretreated with peptide YY at different concentration were stimulated with LPS. The levels of tumor necrosis factor-a （TNF-α） and interleukin-6 （IL-6） in culture supernatant were determined with enzyme-linked im- munosorbent assay. The mRNA expression of TNF-α and IL-6 were detected with PCR. The result showed that the LPS-induced TNF-α production in mouse peritoneal macrophage was suppressed by higher concentration of PYY1-36（10^-8-10^-7 mol/L） or PYY3-36（10^-8-10^-7 mol/L） （P〈0.05）; the LPS-indueed IL-6 production in mouse peritoneal macrophage was suppressed by PYY3-36 at wide rangeof concentration（10^-8-10^-7 mol/L） （P〈0.05）,but not PYY1-36 （P〉0.05）. Our results provide evidence that PYY inhibits to some degree the secretion of pro-inflam- matory cytokines TNF-α and IL 6 in mouse peritoneal macrophage. These data suggest that PYY may inhibit occurrence and development of inflammatory diseases by suppressing secretion of pro- inflammatory cytokines.

关 键 词：PYY 巨噬细胞 炎性细胞因子 小鼠 

分 类 号：S852.21[农业科学—基础兽医学]