连翘酯苷诱生干扰素-γ对AAⅠ诱导的RTECs转分化的影响  

Effect of forsythiaside on aristolochic acid-induced transdifferentiation of renal tubular epithelial cells by up-regulating IFN-γ

作　　者：赵海焦[1] 田娅慧[1] 候晓林[1] 高磊[1] 吴国娟[1] 张中文[1] 

机构地区：[1]北京农学院动物科学技术学院,北京102206

出　　处：《中国兽医学报》2014年第4期613-617,632,共6页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(31172362;30972215);北京市自然科学基金资助项目(5102014)

摘　　要：体外培养小鼠RTECs,将其随机分为6组,CCK-8法测连翘脂苷对RTECs的增殖率及存活率的影响,ELISA检测转化生长因子β1(TGF-β1)和干扰素-γ的含量,RT-PCR和Western blot检测CK18和Smad7等相关因子的表达变化。结果显示连翘酯苷质量浓度为160mg/L以下对RTECs无毒性,且诱生干扰素2.392μg/L;用药E组与模型B组比较TGF-β1表达显著下降(P<0.01);用药组与模型组相比Smad7mRNA极显著高表达(P<0.01)、CK18mRNA显著高表达(P<0.05),而vimentin、α-SMA mRNA抑制表达(P<0.01);Western blot结果表明上调了Smad7的表达量。试验表明连翘脂苷可以提高Smad7的表达量,阻止Smad2/Smad3磷酸化,抑制TGF-β1/Smads信号通路,有效改善AAI诱导的肾小管上皮细胞转分化。In vitro RTECs were randomly divided into six groups. Using CCK-8 assay detected cy- totoxicity of forsythiaside,protein contents of TGF-β1 and IFN-7 in the culture media were exam- ined by ELISA,CK18 and Smad7 expression was detected with RT-PCR and Western blot. The re- sult shows that forsythiaside was non-toxic under 160 mg/L;the expression of TGF-β1 protein was distinctly decreased（P〈0.01）in group E compared with group B; compared with group AAN, Smad7,CK18 mRNA were contrary, vimentin, α-SMA higher expression in treatment groups （P〈0.01, P〈0.05）, on the mRNA were lower expression（P〈0.01） ; western blot showed that forsythiaside enhanced the expression of Smad7. Forsythiaside enhanced the expression of Smad7 by up-regulating IFN-γ, inhibited the TGF-β1/Smad pathway through the restriction of Smad2/ Smad3 phosphorylation,improve the RTECs trans differentiation induced by AAI effectively.

关 键 词：连翘脂苷 肾小管上皮细胞 SMAD7 TGF-β1/Smads信号通路 

分 类 号：S859.7[农业科学—临床兽医学]