梅山猪耳成纤维细胞体外培养及其克隆  

In vitro culture of Meishan pig ear fibroblast cells and cloning

作　　者：张德福[1,2] 吴彩凤[1,2] 戴建军[1,2] 张树山[1,2] 张廷宇[1,2] 王勃 顾晓龙[1,2] 李翔[1,2] 徐利[1,2] 李芳芳[1,2] 韩晓[1,2] 

机构地区：[1]上海市农业科学院畜牧兽医研究所,上海201106 [2]上海市农业遗传育种重点实验室动物遗传工程研究室,上海201106 [3]上海市梅山猪育种中心,上海201800

出　　处：《中国兽医学报》2014年第7期1188-1190,1204,共4页Chinese Journal of Veterinary Science

基　　金：上海市科委农业科技成果转化项目(123919N0700;103919N1800);国家转基因生物新品种培育科技重大专项(2008ZX08006-005;2009ZX08006-014B);上海市农业科学院青年基金资助项目(2011-07)

摘　　要：为获得适合于克隆梅山猪的供体细胞,本试验对梅山猪耳皮肤成纤维细胞的分离、培养、纯化、生长特征和核型分析等进行了研究。通过组织块培养法获得梅山猪皮肤成纤维细胞,用胰酶轻度消化后传代3~4次可得到较纯的皮肤成纤维细胞,并以此作为供体细胞,获得1头健康梅山克隆猪。In order to get appropriate nuclear donor cells for Meishan pig cloning,its ear fibroblast cells were investigated on separation,culture,purification,growth character and karyotype.Meishan pig ear skin fibroblast cells can be obtained by tissue culture and enzymatic digestion purified fibroblast cells after 3-4passages.And one cloned Meishan pig was produced by this cell.

关 键 词：梅山猪 耳成纤维细胞 体外培养 克隆 

分 类 号：S814[农业科学—畜牧学]