菜蛾盘绒茧蜂畸形细胞TATA结合蛋白基因的克隆与序列分析  

Cloning and Sequence Analysis of TATA-binding Protein from the Teratocytes of Cotesia vestalis

作　　者：潘璟[1] 谷启娟 时敏[1] 陈学新[1] 

机构地区：[1]浙江大学昆虫科学研究所/农业部农业昆虫学重点实验室,杭州310058

出　　处：《中国生物防治学报》2014年第3期420-426,共7页Chinese Journal of Biological Control

基　　金：国家"973"计划项目(2013CB127600)

摘　　要：TATA结合蛋白(TATA-binding protein,TBP)及其相关因子是转录起始复合物TFIID的重要组分,能识别TATA元件并与之特异性结合,进而指导RNA聚合酶和其它转录因子有序装配,形成稳定的转录起始复合物。本文采用RT-PCR和RACE技术克隆获得了菜蛾盘绒茧蜂Cotesia vestalis畸形细胞TATA结合蛋白(CvT-TBP)基因的cDNA序列(GenBank No.JX984954),序列全长6105 bp,开放阅读框5784 bp,编码1927个氨基酸,预测分子量213.8 kDa,理论等电点6.82。氨基酸序列分析表明,CvT-TBP与其他膜翅目昆虫的TBP有很高的同源性,其中与切叶蜂Megachile rotundata、小蜜蜂Apis florea和佛罗里达弓背蚁Camponotus floridanus的相似性均为72%。本研究克隆得到的畸形细胞TATA box结合蛋白cDNA序列,为进一步研究该基因功能以及畸形细胞的作用机理提供了基础数据。The TATA-binding protein （TBP） and other associated factors are the central elements of the transcription factor, TFIID. The TATA-binding protein recognizes the TATA sequence and specifically binds to it, which recruits RNA polymerase and other transcription factors to form a stable transcription initiation complex. Here we cloned a TATA binding protein gene of Cotesia vestalis teratocyte （CvT-TBP） （GenBank No. JX984954） using RT-PCR and RACE methods. The full length of this cDNA fragment is 6105 bp, containing an open reading frame of 5784 bp which encodes 1927 amino acid residues with a predicted molecular weight of 213.8 kDa and isoelectric point of 6.82. Homology analysis using BlastP showed that the amino acid sequence of CvT-TBP had a high similarity with those of TBPs from other insects, sharing 72% identity with those of Megachile rotundata, Apis florea and Camponotus floridanus. The results provide a basis for further study of the functional mechanism of teratocytes.

关 键 词：菜蛾盘绒茧蜂 畸形细胞 TATA结合蛋白 基因克隆 序列分析 

分 类 号：S476.3[农业科学—农业昆虫与害虫防治]