外源过氧化氢对菜蛾盘绒茧蜂抗氧化酶和子代滞育的影响  

Effect of Exogenous Hydrogen Peroxide on the Antioxidant Enzymes in Cotesia vestalis and Diapause of Their Progeny

作　　者：贺宜鹏 陈超[1] 柴跑 施祖华[1] 

机构地区：[1]浙江大学昆虫科学研究所,杭州310029

出　　处：《中国生物防治学报》2014年第4期446-452,共7页Chinese Journal of Biological Control

基　　金：农业部"948"项目(2011-G4);国家"973"计划项目(2009CB119005);国家公益性行业(农业)科研专项(201103021)

摘　　要：已有研究表明,菜蛾盘绒茧蜂Cotesia vestalis(Haliday)随饲养的光周期延长,预蛹体内过氧化氢(hydrogen peroxide,H2O2)含量升高,而子代滞育率下降。为探明H2O2在滞育诱导过程中的作用,我们在短光周期下饲养获得的预蛹体外用外源H2O2点滴处理,随后测定预蛹体内的H2O2含量、超氧化物歧化酶(superoxide dismutase,SOD)、过氧化物酶(peroxidase,POD)和过氧化氢酶(calalse,CAT)的活性、子代滞育率。结果表明,外源H2O2点滴处理后的预蛹,其子代在13℃短光周期下的滞育率下降。当用1.6 mol/L H2O2处理预蛹后,子代的滞育率从对照的83.76%下降至35.22%,接近于母代饲养在长光照周期下的滞育率(12.24%);H2O2含量随着外源H2O2点滴处理浓度的升高而升高,变化趋势与随饲养光周期延长而升高的变化趋势一致;SOD活性随着外源H2O2点滴处理浓度的升高而下降,CAT活性随着外源处理的H2O2浓度的升高而升高,二者的变化趋势与随饲养光周期延长而升高/下降的变化趋势相反;POD活性在低浓度(0.2 mol/L)处理时与对照无显著差异,在高浓度(1.6 mol/L)处理时,在处理后的4 h与对照无显著差异,甚至更低,在处理后的20 h则显著升高。表明外源H2O2处理,在滞育的诱导过程中有类似于在长光周期下饲养的作用,能阻止子代滞育的启动,但在对体内各种酶的作用上可能存在差异。It has been reported that, with the prolonged photophase in the photoperiod experienced, the content of hydrogen peroxide （H2O2） in the prepupae ofCotesia vestalis（Haliday）increased, while the diapause incidence in their progeny decreased. In order to further explore the function of H2O2 in the diapause induction process ofC. vestalis, we treated the prepupae obtained under a short photoperiod of 8L：16D with different concentration of H2O2 and measured the content of hydrogen peroxide and activity of superoxide dismutase （SOD）, peroxidase （POD） and catalase （CAT）, as well as the diapause incidence in their progeny. The results showed that when the prepupae were treated with exogenous H2O2, the diapause incidence of their progeny declined when they were reared under a short photoperiod at 13 ℃. The diapause incidence in the progeny generation decreased from 83.76% in the control group to 35.22% in the group treated with 1.6 mol/L exogenous H2O2, close to the diapause incidence （12.24%） of their progeny whose maternal generation were reared under a long photoperiod. The content of H2O2 in the prepupae increased with the increasing concentration of applied exogenous H2O2, similar to the effect of prolonged rearing photoperiod; while the activities of SOD decreased and those of CAT in the prepupae increased with the increasing concentration of exogenous H2O2, just opposite to the effects with increased rearing photoperiod.The POD activity showed no significant difference between the prepupae treated at a low exogenous H2O2 concentration （0.2 mol/L） and the control. However, when the prepupae were treated at a high concentration （1.6 mol/L）, the POD activity was the same as or even lower than the control at 4 h post-treatment, but was much higher at 20 h after treatment. The results indicated that exogenous H2O2 treatment may play a similar role as the long photoperiod in the process of diapause induction inC. vestalis, by preventing diapause occurrence, but has a different way in t

关 键 词：菜蛾盘绒茧蜂 过氧化氢 抗氧化酶 滞育 母代效应 

分 类 号：S476.3[农业科学—农业昆虫与害虫防治] Q965[农业科学—植物保护]