壳寡糖和钕复合处理诱导黄瓜对枯萎病的抗性  

Effects of Chitosan Oligosaccharides in Combination with Neodymium on Resistance of Cucumbers to Fusarium Wilts

作　　者：朱英波[1] 史凤玉[1] 张瑞敬 吴楠[1] 宋士清[1] 

机构地区：[1]河北科技师范学院生命科技学院,秦皇岛066600 [2]张家口市植物保护植物检疫站,张家口075000

出　　处：《中国生物防治学报》2014年第4期528-533,共6页Chinese Journal of Biological Control

基　　金：河北省自然科学基金(C2014407021);河北科技师范学院博士启动基金(2007YB010)

摘　　要：以"津研四号"黄瓜为试材,研究了壳寡糖和钕Nd3+对黄瓜幼苗的诱导抗病性及对枯萎病的防治效果。结果表明,50 mg/L壳寡糖和10 mg/L Nd3+复合处理的相对防效达74.7%,比50 mg/L壳寡糖和10 mg/L Nd3+的单独处理分别提高22.0%和43.8%。分析比较壳寡糖和Nd3+单独和复合处理黄瓜幼苗后,黄瓜根部防御反应相关酶系苯丙氨酸解氨酶(PAL)、过氧化物酶(POD)、多酚氧化酶(PPO)、β-1,3-葡聚糖酶(GLU)比活性的变化趋势,发现壳寡糖和Nd3+复合处理黄瓜植株根部PAL、POD、PPO和GLU等防御酶活性呈上升趋势。研究表明,壳寡糖和Nd3+复合处理能诱导黄瓜幼苗对枯萎病的抗性,且诱导黄瓜抗病性的产生与激活黄瓜相关防御酶的活性有关。Control efficacies of chitosan oligosaccharides in combination with neodymium against Fusarium wilt in cucumber （cv. Jinyan 4） were investigated. Results indicated that control efficacies of 50 mg/L chitosan oligosaccharides in combination with 10 mg/L neodymium were 74.7%, 22.0% and 43.8% higher than those resulted from treatments with 50 mg/L chitosan oligosaccharides and 10 mg/L neodymium alone. The influence of chitosan oligosaccharides and neodymium in single and combinative treatment on activities of defense enzymes of cucumber seedlings were analyzed. Results demonstrated that chitosan oligosaccharides in combination with neodymium increased the activities of phenylalanine ammoialyase, peroxidase, polyphenol oxidase,β-1,3-glucanase of cucumber seedling roots.

关 键 词：壳寡糖 钕 抗病性 防御酶 黄瓜 

分 类 号：S476[农业科学—农业昆虫与害虫防治]