干细胞培养微流控芯片培养区与剪切力有关的分析与设计  

Analysis and Design on Culturing Area of Microfluidic Chip for Culturing Stem Cells Focusing on Shear Force

作　　者：王桐[1] 陈涛[1] 左铁钏[1] 

机构地区：[1]北京工业大学激光工程研究院,北京100124

出　　处：《北京工业大学学报》2014年第4期481-487,共7页Journal of Beijing University of Technology

基　　金：国家自然科学基金资助项目(50875007);国家重大科学仪器专项(2011YQ030112)

摘　　要：针对剪切力是芯片上影响干细胞培养的重要因素,其大小主要由培养基的流速梯度决定,利用realizableκ-ε有限元模型,对8种培养区结构进行了流体力学仿真,得出了培养区内的流速分布,并计算剪切力;通过对比分析,按照减小剪切力、减少液体残留2条要求,从8种结构中筛选出1种适合为干细胞培养提供培养液的结构.根据设计结果总结出2条设计经验.Shear force is of significance for culturing stem cells on the biochip, which depends mainly on velocity gradient of the culture medium. Based on fluid dynamics, eight types of culturing areas were studied using finite element method and realizable κ-ε model in this paper. Distribution of velocity in culturing area and shear force in the center area were obtained. By comparing analysis results, one was selected from eight types of culturing areas according to the requirements of minimizing shear force and remaining less old medium, which were fitful for providing medium for stem ceils on microfluidic chip. According to the results, two pieces of design experience were summed up.

关 键 词：干细胞 微流控 生物芯片 有限元法 

分 类 号：Q813[生物学—生物工程] TH79[机械工程—仪器科学与技术]