温针灸对兔膝关节退变模型血清MMP-1和MMP-13水平的影响  

Effects of Needle Warming Moxibustion on Serum MMP-1,MMP-13 Level in the Knee Osteoarthritis of Rabbits

作　　者：刘娣[1] 武永利[2] 张艳玲[1] 许建峰[2] 马晓秀 路宗志[2] 

机构地区：[1]宁夏医科大学,银川750004 [2]宁夏医科大学总医院中医骨伤科,银川750004

出　　处：《宁夏医科大学学报》2014年第5期497-499,504,F0003,共5页Journal of Ningxia Medical University

基　　金：国家自然科学基金(81260545)

摘　　要：目的观察温针灸治疗兔膝关节退变模型前后血清基质金属蛋白酶1、13(MMP-1,MMP-13)及软骨形态的变化,探讨温针灸治疗膝骨性关节炎的作用机理。方法 30只雄性兔随机分为模型组、温针灸组和西药组,每组10只,采用右后肢伸直位固定制作兔膝关节退变模型。采用双抗体夹心酶联免疫吸附法(ELISA)检测造模前、治疗前和治疗后兔血清中MMP-1,MMP-13水平,参考彭太平法观察膝关节组织形态学变化。结果治疗2周后,大体和光镜观察模型组膝关节软骨变薄,出现明显的关节软骨损伤,温针灸组可明显改善关节软骨组织学结构。各组治疗前较造模前血清MMP-1、MMP-13的浓度均显著升高(P<0.05);治疗两周后,与模型组比较,温针灸组MMP-1、MMP-13水平降低(P<0.05),但与西药组比较,差异无统计学意义(P>0.05)。结论温针灸能治疗KOA的软骨损伤,可能与降低MMP-1和MMP-13表达有关。Objective To investigate the influence of needle warming moxibustion on matrix metalloprotenases and histomorphology of the rabbit experimental knee osteoarthritis model, and to explore its relative mecha- nism. Methods 30 male rabbits were randomly divided into model, needle warming moxibustion and western medicine group, each group of 10 cases. The concentrations of MMP - 1, MMP - 13 in serum were detected by ELISA after two weeks treatment. Results The cartilage became thinning and appeared a significant articular injury. Needle warming moxibustion group can improve the histological structure of articular cartilage. MMP - 1 and MMP - 13 before treatment were increased significantly compared with normale （ P 〈 0.05 ）. Compared with model group,both MMP- 1 and MMP- 13 levels in warm acupuncture- moxibustion group were reduced considerablly （ P 〈 0.05 ）. No significant differences were found between western medication group and model group, western medication group and needle warming moxibustion group. Conclusion MMP - 1, MMP - 13 participated in the occurrence of KOA and needle warming moxibustion can treat KOA ar- ticular injury, which may related with the expression of MMP - 1 , MMP - 13.

关 键 词：膝骨性关节炎 温针灸 MMP-1 MMP-13 

分 类 号：R245[医药卫生—针灸推拿学]