钙蛋白酶对控制通气大鼠膈肌组织学的影响  

The Effect of Calpain on the Morphology of Rat Diaphragm Fibers by Ventilator

作　　者：石磊[1] 郭虹[1] 黄佳茹 马少林[2] 朱晓萍[2] 

机构地区：[1]宁夏医科大学,银川750004 [2]同济大学附属上海东方医院,上海200120

出　　处：《宁夏医科大学学报》2014年第7期725-727,735,F0002,共5页Journal of Ningxia Medical University

基　　金：国家自然科学基金(81170074)

摘　　要：目的观察钙蛋白酶对控制通气膈肌组织形态学的影响,以探讨机械通气导致的膈肌功能异常可能的机制。方法 SD大鼠18只随机分为对照组、24h控制通气组(CMV组)和钙蛋白酶抑制剂组(calpeptin,CMVC组),观察各组大鼠膈肌组织学和超微结构的变化。结果 (1)光镜下显示CMVC组膈肌纤维横截面形状规则,毛细血管内皮略有肿胀,与CMV组相比,未见炎性细胞浸润和中央核的改变;CMVC组膈肌纤维异常组织和结缔组织显著低于CMV组(P<0.05)。(2)电镜下,与CMV组相比,CMVC组膈肌肌原纤维排列整齐,Z线清晰,未见线粒体空泡变;CMVC组膈肌纤维损伤密度显著低于CMV组(P<0.05)。结论钙蛋白酶抑制剂能够明显逆转控制通气所致的膈肌损伤,提示钙蛋白酶可促进通气所致膈肌损伤。Objective To clarify the mechanisms of calpain on morpbological properties of diaphragm fibers under controlled mechanical ventilation in rats. Methods group） were randomly divided into control group, 24 hours A total of 18 Sprague Dawley rats （6 in each of CMV group and treatment with 4mg/kg calpep- tin group （CMVC）. Variants of diaphragm uhrastructure and light microscopic feature were observed. Results （ 1 ） There was a little swelling of capillary endothelial cells in CMVC by light microscopic. Abnormal via- ble, necrotic mass of inflammatory cells and swelling of capillary en,lothelial cells could not be observed. Compared with CMV, the proportion of abnormal muscle and connectiw~ tissue were significantly lower in CM- VC （ P 〈 0.05 ）. （2） Sarcomeres from CMVC remained in register with adjacent sarcomeres and Z - band re- mained relatively intact in diaphragm, atrophic mitochondria could not be seen in CMVC under electron micro- graphs. Compared with CMV, the density of muscle injury was significantly lower in CMVC （ P 〈 0.05 ）. Conclusion Calpeptin reversed the effects of CMV - induced diaphragm injury,it was that calpain contribu- ted the ventilator- induced diaphragm dysfunction.

关 键 词：机械通气 膈肌 钙蛋白酶 肌损伤 

分 类 号：R565[医药卫生—呼吸系统]