GM6001对涎腺腺样囊性癌细胞粘附、侵袭及迁移能力的影响  

Effects of GM6001 on Cell Migration,Adhesion and Invasion in Salivary Adenoid Cystic Carcinoma

作　　者：徐佳[1] 丁晓梅[1] 黑玉娜 铁铃 景捷[2] 

机构地区：[1]宁夏医科大学,银川750004 [2]宁夏医科大学总医院口腔颌面外科,银川750004

出　　处：《宁夏医科大学学报》2014年第7期763-767,F0003,共6页Journal of Ningxia Medical University

摘　　要：目的 研究GM6001对涎腺腺样囊性癌（salivary adenoid cystic carcinoma,SACC）肺高转移性细胞（ade-noid cystic carcinoma-M,ACC-M）和肺低转移性细胞（Adenoid cystic carcinoma-2,ACC-2）的粘附、侵袭及迁移能力的影响.方法 选取处于对数期生长的ACC-M和ACC-2细胞,应用MTT法测定不同浓度的GM6001（10、15、20、25 μmol·L-）对ACC-M和ACC-2细胞干预前后粘附能力的影响;应用Transwell小室通过平均穿膜细胞数观察不同浓度的GM6001对ACC-M和ACC-2细胞干预前后侵袭及迁移能力的影响.结果 GM6001在10～25μmol·L-的范围内可抑制ACC-M与ACC-2细胞的粘附能力,ACC-M细胞粘附实验的OD值从（0.226±0.196）降低至（0.065±0.010）;ACC-2细胞的（0.265±0.026）降低至（0.122±0.007）.GM6001浓度在0～25 μmo·lL-的范围内干预ACC-M与ACC-2细胞后侵袭至小室下的细胞数分别从（42.00±2.550）个降至（20.40±3.435）个、（40.20±3.033）个降至（22.00±2.550）个;迁移至小室下的细胞数分别从（62.20±3.768）个降至（17.60±3.847）个、（55.40±2.302）个降至（18.20±2.387）个,GM6001对ACC-M细胞粘附、侵袭及迁移的抑制能力强于对ACC-2细胞的抑制能力.结论 GM6001能降低ACC-M及ACC-2细胞的粘附、侵袭及迁移能力,其在SACC临床治疗中可能具有应用前景.Objective To investigate the effects of GM6001 on the SACC of high pulmonary metastatic cell line ACC - M and low metastatic lung cell line ACC - 2 of adhesion, invasion and migration introduced. Methods The logarithmic phase cells of ACC - M and ACC - 2 were selected and the effects of different con- centration of GM6001 （ 10,15,20,25） μmol · L - 1 ） on the ACC - M and ACC - 2 cellg adhesion were analyzed by MTI＂ essay. The Transwell chamber was used to observe the influence of GM6001 in the invasion and mi- gration. Results GM6001 could inhibit the adhesion ability of ACC - M and ACC - 2 cell in the range of10- 25 μmol · L-L. The ACC -M cells adhesion inhibition of OD value was from （0.226± 0. 196）reduces to （0. 065 ±0. 010）. The ACC -2 cells adhesion inhibition of OD value was from （0.265±0. 026） reduces to （0. 122 ±0. 007）. The Concentration of GM6001 in the range of 0 μmol · L^-1 intervention ACC -M and ACC - 2 cells invaded to the small room next to the number of cells were （42.00 ± 2. 550） - （ 20.40 ± 3.435）, （40.20 ±3. 033 ） -（22. 00 ±2. 550）. Migrated to a small room next to the number of cells were （62.20±3.768）-（17.60±3.847）, （55.40±2.302） -（18.20±2.387）. Conclusion GM6001 has the capability to restrain ACC -M cells and ACC -2 cells from adherinlg, invasion and migration and it may has application prospect in clinic treatment.

关 键 词：涎腺腺样囊性癌 金属蛋白酶组织抑制剂 粘附能力 侵袭能力 迁移能力 

分 类 号：R739.87[医药卫生—肿瘤]