马尔尼菲青霉菌内源启动子驱动的苯菌灵抗性基因盒构建及其应用  

Construction of the Benomyl Resistance Gene Cassette Driven by the Endogenous Promoter and its Use in Penicillium marneffei

作　　者：卢姗[1] 毛从政 陶玉婷[1] 黄勇奇[1] 贺菽嘉[1] 梁莹[3] 梁晓乐[4] 吴谦[1] 何志义[2] 蓝秀万[1,4,5] 

机构地区：[1]广西医科大学基础医学院生物化学与分子生物学教研室,南宁530021 [2]广西医科大学第一附属医院呼吸科,南宁530021 [3]广西医科大学基础医学院微生物学教研室,南宁530021 [4]广西医科大学基础医学中心实验室,南宁530021 [5]广西艾滋病防治研究重点实验室,南宁530021

出　　处：《基因组学与应用生物学》2014年第2期253-259,共7页Genomics and Applied Biology

基　　金：国家自然科学基金项目(项目编号:81160195);广西自然科学基金项目(项目编号:桂科青0991031)共同资助

摘　　要：马尔尼菲青霉菌是一种区域性流行于东南亚及中国南部地区的条件致病性温度依赖性双相型病原真菌,它能对免疫功能低下者造成致命的全身性感染,25℃时呈现菌丝状生长,而在37℃或在宿主体内为酵母相生长。为深入研究马尔尼菲青霉菌致病性和双相转化的分子机制,原有的遗传转化筛选标记基因已不能满足研究需要。为此,我们通过用马尔尼菲青霉菌微管蛋白β亚基基因启动子替换粗脉孢菌苯菌灵抗性基因的启动子,构建了一个苯菌灵抗性基因盒,并成功地将其运用于马尔尼菲青霉菌的遗传转化研究中。Penicillium marneffei is an opportunistic fungal pathogen endemic in Southeast Asia and southern China, causing lethal systemic infections in immunocompromised patients. P. marneffei grows in a mycelia form at the ambient temperature of 25℃, and in a yeast form at 37℃. In order to research into the molecular mechanism of pathogenicity and phase transition in P. marneffei, more efficient genetic selectable markers need to be developed. In this research, we developed a new benomyl resistance gene cassette by replaced the promoter of the benomyl resistance gene of Neurospora crassa with the promoter of β-tubulin gene of P. marneffei. This benomyl resistance gene cassette has been successfully used as a dominant genetic selectable marker in P. marneffei.

关 键 词：马尔尼菲青霉菌 苯菌灵抗性基因盒 遗传转化 

分 类 号：R378[医药卫生—病原生物学]