凋亡相关基因PDCD5的表达与桥本甲状腺炎临床相关性的研究  

Correlation between Hashimoto's Thyroiditis and Expression of Apoptosisrelated Gene PDCD5

作　　者：赵杰[1] 李超[1] 赵丽娜[1] 

机构地区：[1]河北联合大学生命科学学院,唐山063000

出　　处：《基因组学与应用生物学》2014年第3期495-500,共6页Genomics and Applied Biology

基　　金：国家自然科学基金项目(39870427);唐山市科技局计划项目(121302021a);河北省高等学校科学技术研究项目(QN20131059)共同资助

摘　　要：本实验旨在探明程序性死亡因子5(programmed cell death 5,PDCD5)在桥本甲状腺炎(hashimoto's thyroiditis,HT)病程中的表达,并研究其与HT疾病发生发展之间的关系。以β-actin基因、β-actin蛋白为内参照,采用RT-PCR法和Western印迹法检测60例HT患者和40例健康对照者外周血单个核细胞(PBMC)PDCD5 mRNA和蛋白的表达水平。以外周血血清中抗甲状腺微粒体抗体(TMAb)水平45%为标准,划分为低TMAb组(TMAb≤45%)和高TMAb组(TMAb>45%),比较两组患者的血清TGAb值、甲状腺激素水平及PDCD5的表达水平。结果表明,与健康对照组相比较HT患者PDCD5 mRNA和PDCD5蛋白的表达阳性率和相对表达水平显著升高(p<0.05),在高TMAb水平组PDCD5的表达更高,较低TMAb水平组有显著性差异(p<0.05),TGAb水平显著高于低TMAb组(p<0.01),血清TT3、TT4、FT3、FT4没有统计学差异(p>0.05)。由于PDCD5的表达量在HT患者PBMC中上调,并且随病情加重其表达量逐渐增加,初步判定在HT疾病的发生发展过程中,PDCD5具有重要意义。The purpose of our experiment is to ascertain the expression of programmed cell death 5（PDCD5） gene in hashimoto＇s thyroiditis（HT） and to explore the effects of apoptosis on the genesis and development of HT. Usingβ-actin and β-actin protein for reference, we detected the expression levels of PDCD5 mRNA and protein in peripheral blood mononuclear cells（PBMC） of 60 HT patients and 40 healthy controls via RT-PCR and Western blot.According to the serum TMAb level, the patients were divided into low TMAb group（TMAb≤45%） and high TMAb group（TMAb〉45%）. Then match the serum TGAb level, thyroid function and PDCD5 expression in PBMC between the two groups. Compare with normal donor group, the positive rate and the relative expression level of PDCD5 was significantly increased in HT groups（p〈0.05）. PDCD5 mRNA and PDCD5 protein in high TMAb group was extraordinary higher than that in low TMAb group（p〈0.05）, so as TGAb level in the low TMAb group（ p〈0.01）, no statistical difference was found in serum TT3, TT4, FT3, FT4（p〈0.05）. The up-regulation of PDCD5 in HT patients and with the aggravation of HT the expression of PDCD5 increased gradually preliminary determination as to promote regulatory factor of lymphocyte apoptosis PDCD5 plays an important role in the pathogenesis of HT.

关 键 词：程序性死亡因子5 桥本甲状腺炎 细胞凋亡 外周血单个核细胞 反转录-聚合酶链式反应 WESTERN印迹法 

分 类 号：R581.4[医药卫生—内分泌]