牛Phlda2基因的组织表达及生物信息学分析  

Tissue Expression and Bioinformatics Analysis of Bovine Phlda2 Gene

作　　者：赵姝君 王敬姣 王梦楠[1] 吴茜红[1] 李冬杰[2] 李世杰[1] 

机构地区：[1]河北农业大学生命科学学院,保定071001 [2]河北科技大学生物科学与工程学院,石家庄050018

出　　处：《基因组学与应用生物学》2014年第3期505-510,共6页Genomics and Applied Biology

基　　金：国家自然科学基金(31372312);河北省自然基金项目(C2011204001)共同资助

摘　　要：在人、鼠和猪中,Phlda2基因是一个母源表达的印记基因,编码一种胞浆蛋白,具有与血小板同源的结构域。在牛中,Phlda2基因的结构和功能还不清楚。本研究首先利用RT-PCR方法对Phlda2基因在牛组织中的表达进行了分析,进而采用生物信息学方法对牛Phlda2基因的分子进化、启动子及蛋白的高级结构进行了分析和预测。结果表明,Phlda2基因在牛的心、肝、脾、肺、肾、大脑、骨骼肌、皮下脂肪、卵巢和胎盘10个组织中均有表达。对包括人、小鼠、牛、猪、原鸡、非洲爪蝉、斑马鱼和袋鼠在内的8种动物Phlda2基因mRNA序列进化进行分析,发现这8种动物的遗传距离小于0.435,且牛和猪遗传距离最近,为0.148,与基因系统进化树分析的结果一致。牛Phlda2基因的启动子最可能位于该基因起始密码子上游487-737bp区域,包括20种转录因子结合位点。牛Phlda2基因编码亲水性蛋白,其二级结构主要以α-螺旋和无规则卷曲为主;蛋白三级结构包括一个由β片层和α-螺旋构成的血小板同源结构域（PH）。以上研究结果将为进一步研究Phlda2基因的功能和分析基因表达调控的分子机理奠定基础。In human, mice and pig, Phlda2 is a maternally expressed imprinted gene and encodes cytoplasm protein with pleckstrin homology domain. In bovine, the gene structure and function of Phlda2 gene have not been studied.In this study, the expression of Phlda2 gene in bovine tissues was firstly analyzed by RT-PCR, then the molecular evolution, the promoter and protein conformation of bovine Phlda2 gene were analyzed using software and on-line tool. The results showed that the Phlda2 gene was expressed in nine detected tissues（heart, liver, spleen, lung, kidney, skeletal muscle, subcutaneous fat, ootheca and placenta）. The genetic distance of 8 species（including human,mouse, bovine, pig, chicken, African frog, zebrafish and kangaroos） was less than 0.435, and bovine and pig shared the minimum（0.148）, which accordant with the results of phylogenetic tree. Using promoter online software combined with transcription factor analysis, the potential promoter of bovine Phlda2 was predicted to locate in 548-737 bp upstream the start codon. Protein software analysis showed that the Phlda2 gene secondary structure is relatively simple including α-turn and random coil. These results may increase our understanding on bovine Phlda2gene and may provide informations to study its function and regulation mechanism.

关 键 词：牛 Phlda2基因 生物信息学 组织表达 

分 类 号：S823[农业科学—畜牧学]