三七及屏边三七病程相关基因克隆的新策略  

A New Strategy of Cloning of Resistance Genes in Panax notoginseng and Panax stipuleanatus

作　　者：李瑞博[1,2] 申业[1] 文国松[3] 冯光泉[4] 曲媛[2] 崔秀明[2] 黄璐琦[1] 

机构地区：[1]中国中医科学院中药资源中心,北京100070 [2]昆明理工大学生命科学与技术学院,昆明650000 [3]云南农业大学中药材研究所,昆明650201 [4]文山学院文山三七研究院,文山663000

出　　处：《基因组学与应用生物学》2014年第3期624-632,共9页Genomics and Applied Biology

基　　金：国家自然基金项目(81360617;81130070;81173490);云南省应用基础研究计划项目(2013FA031)共同资助

摘　　要：三七[Panax notoginseng(Burk)F.H.Chen]是我国传统名贵中药材之一。近年来,三七的病虫害问题变得越来越严峻,现已成为限制三七产业可持续发展的一个重要因素。观察发现,作为三七的近缘物种屏边三七[Panax stipuleanatus H.T.Tsai et K.M.Feng]在其相同的生长环境下抗病性状表现优异。如何从分子角度挖掘利用植物本身所具备的抗病基因必将成为三七未来研究当中的一个热点问题。本研究利用人参、西洋参的EST数据库,通过基因注释、KEGG作图找到病原与植物识别代谢途径上有关植物病程相关的基因。然后经RT-PCR和TA克隆分别获得了若干个三七和屏边三七病程相关基因,该技术的应用为三七抗病基因的研究和克隆开辟了新的技术途径,同时也对其他药用植物的抗病研究具有参考意义。Panax notoginseng is a traditional Chinese famous medicinal material. It is an undeniable fact that the disease problem becomes increasingly serious. Now the disease has become an important factor to limit industrial sustainable development. It was observed the disease resistant trait of Panax stipuleanatus is better than notoginseng in equivalent environment. It would be one of the hot topics to cloning resistant gene. Some resistance genes of plant-pathogens interaction in Panax notoginseng and stipuleanatus were cloned by homology PCR based on gene annotation and KEGG analysis data of related species. This method is a new way to clone gene of notoginseng, meanwhile, the research is also a reference to other medicinal plant of pathological research.

关 键 词：三七 屏边三七 抗病基因 电子克隆 

分 类 号：S435.672[农业科学—农业昆虫与害虫防治]