二倍体与四倍体葡萄品种杂交胚挽救及后代倍性鉴定  

Rescue of Embryos from Cross Between Diploid and Tetraploid Grape Cultivars and Their Ploidy Identification

作　　者：郭印山[1] 陈文玲[1] 郭修武[1] 刘镇东[1] 林洪[1] 唐健[1] 

机构地区：[1]沈阳农业大学园艺学院,沈阳110161

出　　处：《沈阳农业大学学报》2014年第3期274-278,共5页Journal of Shenyang Agricultural University

基　　金：国家现代农业产业技术体系项目(CRAS-30-yz-6);国家自然科学基金项目(31000894;31372021);辽宁省重大科技攻关项目(2011204001);高等学校博士点基金项目(20102103120003);辽宁省教育厅基金项目(L2010492);沈阳市人才资源开发专项项目(2011)

摘　　要：以葡萄二倍体品种无核红宝石与四倍体品种香悦为亲本进行正反杂交,以杂交胚珠为试材进行胚挽救试验,研究不同取样时期对胚珠萌发率的影响,采用流式细胞仪法与染色体计数法对杂交后代幼苗倍性进行鉴定。结果表明:无核红宝石×香悦于花后60d接种胚珠萌发率最高,为8.33%,获得杂种胚挽救幼苗8株,其中有1株三倍体植株(12.5%);香悦×无核红宝石在花后83d接种胚珠萌发率最高,为29.38%,获得杂种胚挽救幼苗56株,其中有16株三倍体植株(27.59%)。In this study, the impact of inoculating stage on ovule germinating rate was investigated using Hybrid ovules from the reciprocal crosses between diploid seedless cultivar ＂Ruby Seedless＂ and tetraploid cultivar ＂Xiangyue＇. Furthermore, the chromosome ploidy of these hybrids was determined by flow cytometry and chromosome counting. The main results were as follows. The highest ovule germinating rate （8.33%） appeared when inoculation was done 60d after flowering for the cross ＂Ruby Seedless＂ x ＂Xiangyue＇, from which 8 seedlings were rescued, among which one seedling was triploid （triploid percentage 12.5%）. For the cross ＂Xiangyue＂ x ＂Ruby Seedless＇, the highest germination rate （29.38%） was obtained when inoculation was done 83d after flowering. 56 seedlings were rescued from this cross, and 16 of them were triploid （27.59%）.

关 键 词：葡萄 二倍体 四倍体 胚挽救 倍性鉴定 

分 类 号：S663.1[农业科学—果树学]