红尾副鳅线粒体基因组序列测定与分析  

Sequencing and analysis of the complete mitochondrial genome of Paracobitis variegatus

作　　者：史妍茹[1] 邢连喜[2] 陈涛[3] 杨敏[1] 尤平[1] 

机构地区：[1]陕西师范大学生命科学学院,陕西西安710119 [2]西北大学生命科学学院,陕西西安710069 [3]桂林理工大学国有资产管理处,广西桂林541004

出　　处：《陕西师范大学学报（自然科学版）》2014年第4期50-56,共7页Journal of Shaanxi Normal University：Natural Science Edition

基　　金：国家自然科学基金资助项目(31372158);中央高校基本科研业务费专项资金项目(GK201002044)

摘　　要：采用长PCR和嵌套PCR相结合的方法测定并注释了红尾副鳅Paracobitis variegatus(Dabry de Thiersant,1874)线粒体基因组全序列.结果表明:红尾副鳅线粒体基因组全长16 571bp,A+T含量55.6%,基因构成及排列与其他硬骨鱼基本相同,包含37个基因和1个控制区(D-loop区).基因组结构紧密,除控制区外基因间仅存在63bp的间隔,33bp的重叠.13个蛋白质编码基因中,除了COI基因以GTG作为起始密码子,COII、COIII和Cyt b以不完整的T,ND4基因以不完整的TA作为终止密码子外,大都以典型的ATG作为起始密码子,以TAA或TAG作为终止密码子.22个tRNA基因的二级结构中,除了tRNASer(AGN)外,其余21个都可以折叠成典型的三叶草结构,tRNASer(AGN)缺失了DHU臂,在相应位置上只形成一个环.预测了rRNA的二级结构,其中12SrRNA包含43个茎环结构,16SrRNA包含6个结构域,53个茎环结构.控制区包含1个终止相关序列TAS1、3个中央保守序列(CSB-F、CSB-D、CSB-E)、2个保守序列区(CSB-2、CSB-3).The complete mitochondrial genome （mitogenome）sequence of Paracobitis variegatus （Dabry de Thiersant, 1874 ） was sequenced using the strategy of sub-PCR after long-PCR amplification and annotated.The results showed that the entire mitochondrial genome of P. variegatus is 16 571 bp long with A＋T content 55.6 !.The mitochondrial genome organization of P.variegatus is the same with that in bony fish,which contains 3 7 genes and 1 control region （named D-loop）.The genomic structure is arranged densely,besides D-loop control region,there is only 63 bp intergenic spacer,and 33 bp overlap between genes.Except for COI gene,which uses GTG as the initiation codon,all other protein-coding genes start with the typical ATG codon.The most of 1 3 protein-coding genes terminated with the typical TAA or TAG codons, while COⅡ,COIII and Cyt b were infered to have an incomplete termination codon T,and ND4 gene terminated with an incomplete termination codon TA.Most tRNA genes could form typical secondary structures except tRNA^Ser （AGN ）, which had an absence of the DHU arm.The secondary structures of rRNA were predicted.There are 43 helices structures in 12S rRNA,and 6 domains,53 helices structures in 16S rRNA.In addition,there are 1 terminal associated sequence named TAS1 ,3 central conserved sequence blocks named CSB-F,CSB-E and CSB-D,2 conserved sequence blocks named CSB-2,CSB-3 in the control region of P.variegatus.

关 键 词：红尾副鳅 线粒体基因组 tRNA二级结构 控制区 

分 类 号：Q951[生物学—动物学]