正交提取双水相体系纯化新楝果核印楝素的工艺研究  

Study on separation of azadirachtin from new neem kernels by orthogonal design mode and aqueous two-phase system

作　　者：王曼[1] 王佳静[1] 栾龙[1] 孙润广[1] 

机构地区：[1]陕西师范大学物理学与信息技术学院,陕西西安710119

出　　处：《陕西师范大学学报（自然科学版）》2014年第4期73-77,共5页Journal of Shaanxi Normal University：Natural Science Edition

基　　金：国家自然科学基金资助项目(10874108);陕西省自然科学基础研究计划(SJ08A16)

摘　　要：采用乙醇-无机盐双水相技术,研究了从国产新楝果核中提取印楝素的工艺.通过单因素实验和正交实验探讨了无机盐的质量浓度、提取时间、料液比对印楝素提取率的影响.实验结果表明:正交提取、双水相体系纯化印楝素的最佳条件是:无机盐浓度为0.62g/mL,提取时间为1.5h,料液比为1∶10(g∶mL),此时印楝素的提取率为1.23%.The separation of azadirachtin from new neem kernels was studied by alcohol-inorganic salt a-queous two-phase extraction technology.The single factor and orthogonal experiments were done to in-vestigate the varitaion of azadirachtin extraction ratio relating to inorganic salt concentration,extraction time and material-solvent ratio.The results showed that the extraction rate of azadirachtin was 1 .2 3%under the optimum condition that the concentration of inorganic salt was 0.62 g/mL,the extraction time was 1 .5 h,and the material-solvent ratio was 1∶1 0 （g∶mL）.

关 键 词：双水相纯化 提取率 印楝素 

分 类 号：Q949.753.1[生物学—植物学]