芒果总DNA提取方法比较分析  

Comparison of different extraction methods of total DNA in Mangifera indica

作　　者：张宇[1,2] 黄国弟[1] 唐志鹏[2] 李剑雄 

机构地区：[1]广西壮族自治区亚热带作物研究所,广西南宁530001 [2]广西大学农学院,广西南宁530004 [3]北京市陶然亭公园管理处,北京100050

出　　处：《经济林研究》2014年第2期62-65,共4页Non-wood Forest Research

基　　金：国家行业(农业)科研专项经费项目(201203092-5);广西自然科学基金(桂科自08320338)

摘　　要：为了研究不同提取方法对芒果总DNA提取质量的影响，以‘紫花芒’等11个芒果品种的叶片为材料，采用SDS法、CTAB法、改良CTAB法提取芒果叶片总DNA，并对提取的总DNA浓度、OD260/280、OD260/230、琼脂糖电泳结果、ISSR扩增产物进行了比较分析。结果表明，改良CTAB法能较好地去除芒果叶片中的多酚、多糖、单宁和色素等杂质。提取的总DNA浓度高达387 ng/μL，OD260/280在1.81～1.87之间，OD260/230在2.01～2.09之间。SDS法、CTAB法提取总DNA的ISSR扩增产物条带少，改良CTAB法提取的总DNA的扩增效果图谱清晰，多态性高。3种提取方法的结果比较而言，以改良CTAB法提取总DNA的效果最好，可用于后续实验的研究。In order to research the effects of different extraction methods on extraction quality of total DNA in Mangifera indica, taking leaves in eleven cultivars of M. indica as materials, total DNA was extracted by the methods of SDS, CTAB and improved CTAB, and the concentration of total DNA, OD260/280, OD260/230, agarose gel electrophoresis test results, ISSR ampliifcation products were compared and analyzed. The results indicate that the improved CTAB method can well remove the impurities in M. indica leaves, for example polyphenol, polysaccharide, tannin, pigment, and so on. The total extracted DNA concentration is up to 387 ng/μL, OD260/280 is from1.81 to 1.87, and OD260/230 is from 2.01 to 2.09. The ISSR productions of total DNA extracted by SDS and CTAB method have fewer bands, but ampliifed bands of total DNA extracted by improved CTAB method is clear and has high polymorphism. In the three methods, the improved CTAB method has the best extraction effect, and it can be used for the subsequent researches.

关 键 词：芒果 CTAB法 

分 类 号：S667.7[农业科学—果树学]