青蒿愈伤组织的诱导及化学成分变化规律研究  

Establishment of rapid callus proliferation culture system and evaluating content of secondary metabolite in Herba Artemisiae Annuae

作　　者：郭新荣[1] 李晋[1] 马琳[1] 王振中[2] 萧伟[2] 常艳旭[1,2] 

机构地区：[1]天津市现代中药重点实验室天津中医药大学,天津300193 [2]江苏康缘药业股份有限公司,连云港222001

出　　处：《天津中医药大学学报》2014年第4期219-224,共6页Journal of Tianjin University of Traditional Chinese Medicine

基　　金："重大新药创制"科技重大专项资助项目(2010ZX09502-005);中国博士后特别基金资助项目(2012T50239);江苏省博士聚集计划;天津市科技支撑计划重大项目(10ZCZDSY12400)

摘　　要：[目的]建立青蒿愈伤组织快速繁殖的适宜培养基,测定其生长过程中次生代谢产物奎宁酸类化合物和香豆素类化合物的含量变化。[方法]无菌条件下切取花序、叶和叶柄为外植体,接种于MS添加不同激素组合的培养基,进行诱导及继代增殖培养,测定奎宁酸类化合物和香豆素类化合物的含量。[结果]建立了青蒿花序、叶和叶柄愈伤组织快速繁殖的适宜培养基,绘制相对应的生长曲线,并确定了生长过程中次生代谢产物奎宁酸类化合物和香豆素类化合物的含量变化及最佳采收期。[结论]通过组织培养方式可以成功获得青蒿愈伤组织生长曲线和生长过程中次生代谢产物的变化规律,对传统中药青蒿培养体系优化及工业化规模生产进行探索提供了一个新途径。[Objective] To establish the rapid callus proliferation culture system of Herba A rtemisiae A nnuae and determine the content of phenylpropionic acids and coumarins. [Methods] The inflorescence, leaf and petiole were cut down and selected as explant and induced into callus under sterile conditions. Then, the callus was planted in MS with different combinations of different hormones. Finally, the content of phenylpropionic acid and coumarins was determined. [Results] The rapid callus proliferation culture system of Herba A rtemisiae A nnuae was established successfully by tissue culture. Kinetics of growth accumulation and the best harvest of phenylpropionic acid and coumarins biosynthesis were evaluated. [Conclusion] Kinetics of growth accumulation of secondary metabolites of A rtemisia A nnue can be successfully established by tissue culture. These methods provide a new way to explore the variation of the secondary metabolites in culture system optimization and industrial scale production of A rtemisia A nnua.

关 键 词：青蒿 组织培养 奎宁酸 香豆素 次生代谢产物 

分 类 号：R285.5[医药卫生—中药学]