类风湿关节炎患者外周血单个核细胞microRNA-206表达水平的变化及意义  

Level of MicroRNA-2 0 6 in Peripheral Blood Mononuclear Cells from Rheumatoid Arthritis and Their Significance

作　　者：徐庆雷[1] 朱宝林[1] 马小波[1] 张巍[1] 刘玲玲[1] 王胜军[2] 陈建国[2] 

机构地区：[1] 沭阳县人民医院检验科,江苏沭阳223600 [2] 江苏大学附属人民医院,江苏镇江212002

出　　处：《现代检验医学杂志》2014年第5期64-66,共3页Journal of Modern Laboratory Medicine

基　　金：江苏省卫生厅科研资助项目（Z201313）.

摘　　要：目的：探讨外周血单个核细胞microRNA-206在类风湿关节炎发生中的作用。方法收集2012年12月-2013年11月确诊的类风湿关节炎患者27例及25例健康对照外周血标本，分离 PBMCs。采用实时定量 PCR（qRT-PCR）方法检测PBMCs中microRNA-206及锌指样转录因子4（KLF4）、孤儿核受体γt（RORγt）mRNA相对表达量。统计学处理采用非配对t检验和 Spearman相关分析。结果类风湿关节炎患者外周血单个核细胞 microRNA-206表达的水平显著低于健康对照组（0.056±0.019 vs 0.138±0.057，t＝3.103，P＜0.01），KLF4 mRNA和RORγt mRNA表达水平显著高于健康对照组（0.604±0.183 vs 0.098±0.027，t＝6.651，P＜0.01；0.583±0.271 vs 0.069±0.018，t＝7.438，P＜0.01），类风湿关节炎患者外周血单个核细胞microRNA-206表达水平与 KLF4及 RORγt相对表达量均呈负相关（r＝-0.639，P＜0.01；r＝-0.842，P＜0.01）。结论类风湿关节炎患者外周血单个核细胞 microRMA-206表达的水平下降，与疾病的发生发展关系密切。Objective To explore the role of microRNA-206 （miR-206）in peripheral blood mononuclear cells （PBMC）from rheumatoid arthritis （RA）patients.Methods 27 patients with RA and 25 healthy controls were enrolled into the current study.Human peripheral blood mononuclear cells （PBMCs）were isolated by standard density-gradient centrifugation over Ficoll-Hypaque solution in rheumatoid arthritis and healthy control volunteers.Total RNAs were extracted from PBMCs which were stimulated by PMA and ionomycin,then the RNA was transcribed reversely into cDNA.The expression of mi-croRNA-206 and Kruppel-like factor 4 （KLF4）and the orphan nuclear receptor RORγt mRNA was detected by real-time quantitative PCR （qRT-PCR）method.Student’s unpaired t-test and spearman correlation were used for statistica1 analysis. Results The expression of miR-206 in the PBMCs of RA patients was significantly decreased compared with that of the healthy controls （0.056±0.019 vs 0.138±0.057,t=3.103,P〈0.01）,The levels of KLF4 and RORγt mRNAin the PB-MCs of RA patients were increased significantly verves those of the healthy controls（0.604±0.183 vs 0.098±0.027,t=6.651,P〈0.01;0.583±0.271 vs 0.069±0.018,t=7.438,P〈0.01）,Furthermore,a negative correlated between the ex-pression of miR-206 and KLF4 or RORγt mRNA in RA patients （r=-0.639,P〈0.01;r=-0.842,P〈0.01）.Conclusion These results indicated that the augmented expression of KLF4 mRNA may be caused by the attenuated expression of miR-206,and the high level of KLF4 mRNA evokes the proportion of Th17 cells in RA patients.

关 键 词：类风湿关节炎 microRNA-206 锌指样转录因子4 

分 类 号：R593.22[医药卫生—内科学]