转录因子TDF1对拟南芥雄性不育突变体atms1的温敏调控机制研究  

Reaserch on temperature-sensitive regulation mechanism of transcription factor TDF1 to thermo-sensitive male sterile mutant atms1 of arabidopsis thaliana

作　　者：郑亚洁[1] 高贝[1] 夏群芳 周树敏[1] 张卫[1] 

机构地区：[1]上海大学生命科学学院 上海市能源作物育种及应用重点实验室,上海200444

出　　处：《上海师范大学学报（自然科学版）》2014年第3期259-265,共7页Journal of Shanghai Normal University(Natural Sciences)

基　　金：国家自然科学基金面上项目(30870225);上海市教委浦汇人才计划(08PJLH05500)

摘　　要：TDF1作为MYB家族的转录因子,参与了胼胝质的降解过程,并控制绒毡层的分化从而影响着花粉粒的正常发育.以一株通过甲基磺酸乙酯(EMS)诱变Col-0野生型拟南芥获得的温敏雄性不育突变体atms1(ambient temperature-sensory male sterility1)对温度敏感机制进行了研究.通过构建TDF1反义RNA表达载体,在atms1突变体背景下抑制TDF1的表达来观察突变体花粉育性的变化,发现在27℃时的atms1突变体花粉的育性得以恢复.这一结果表明在花粉发育的过程中TDF1对ATMS1m具有一定的调控作用.TDF1 is a transcription factor of MYB family. It is involved in the process of callose degradation and regulating the ta- petum differentiation, thus affecting the normal development of pollen. This paper investigates the temperature - sensitive mecha- nism of an Arabidopsis male sterile mutant atms1 （ ambient temperature - sensory male steilityl ） obtained by screening in the progeny generations of wild- type Col -0 mutagenized by EMS. TDF1 Antisense RNA vector was introduced into atmsl back- ground to inhibit the expression of TDF1 to observe the changes of atmsl mutant pollen fertility. We found that the fertility was re- stored at 27 ℃. The results show that TDF1 may have regulatory effects on ATMS1m in the process of pollen development.

关 键 词：atms1 温敏雄性不育 TDF1 花药育性 Real—time PCR 

分 类 号：Q94[生物学—植物学]