宫颈腺癌合并鳞状上皮内瘤变人乳头状瘤病毒亚型感染与p16蛋白表达的关系  

Relationship between human papillomavirus subtypes infections in cervical adenocarcinoma patients complicated with squamous intraepithelial neoplasia and expression of p16 protein

作　　者：何惠华[1] 刘汉忠[1] 涂宁芳 李蓓[1] 杨继洲[1] 敖启林[3] 

机构地区：[1]华中科技大学同济医院附属孝感市中心医院病理科,湖北孝感432000 [2]孝感市第一人民医院病理科,湖北孝感432000 [3]华中科技大学同济医学院附属同济医院病理研究所,湖北武汉430030

出　　处：《中华医院感染学杂志》2014年第23期5799-5801,共3页Chinese Journal of Nosocomiology

基　　金：国家自然科学基金资助项目(81270105)

摘　　要：目的探讨宫颈腺癌发生鳞状上皮内瘤变(CIN)中人乳头状瘤病毒(HPV)感染及其亚型特点,并探讨p16蛋白在宫颈腺癌组织或CIN病变组织的表达。方法收集2010年1月-2012年12月宫颈腺癌病例66例,对发生CIN患者利用表面等离子体谐振技术进行HPV基因分型检查,免疫组织化学检测p16蛋白,采用SPSS 13.0分析。结果宫颈腺癌发生CIN 21例患者HPV感染率为100.0%,HPV 16型7例为33.3%、HPV 18型6例为28.6%和HPV52型3例为14.3%,18例p16蛋白阳性患者中宫颈腺癌组织及CIN病变组织内均为强阳性。结论宫颈腺癌发生CIN中的宫颈腺癌组织及CIN病变组织均与HPV感染关系密切,HPV16型感染较宫颈腺癌不伴CIN略多见,与p16蛋白表达密切相关。OBJECTIVE To explore the subtypes of human papillomavirus （HPV） causing infections in patients with cervical adenocarcinoma complicated with squamous intraepithelial neoplasia and study the expression of p 16 pro‐tein in the cervical adenocarcinoma tissues or cervical intraepithelial neoplasia tissues . METHODS Totally 66 patients with cervical adenocarcinoma who were treated in the hospitals from Jan 2010 to Dec 2012 were enrolled in the study ,including 21 patients complicated with cervical intraepithelial neoplasia ,then the genotypes of HPV were examined by using surface plasmon resonance technique ,the p16 protein was detected by means of immuno‐histochemistry method ,and the statistical analysis was performed with the use of SPSS13 .0 software .RESULTS The incidence of HPV infections was 100 .0% in the 21 cervical adenocarcinoma patients complicated with cervical intraepithelial neoplasia ,among whom there were 7 （ 33 .3% ） cases of HPV 16 infection ,6 （28 .6% ） cases of HPV 18 infection ,and 3 （41 .3% ） cases of HPV52 infection .The cervical adenocarcinoma tissues and cervical in‐traepithelial neoplasia tissues showed intensively positive for p16 protein in 18 patients .CONCLUSION The HPV infections are closely associated with the cervical adenocarcinoma tissues and cervical intraepithelial neoplasia tis‐sues of the cervical adenocarcinoma patients complicated with cervical intraepithelial neoplasia .The HPV16 infec‐tion is more common in the cervical adenocarcinoma patients complicated with cervical intraepithelial neoplasia than in those without cervical intraepithelial neoplasia ,which is closely related to the expression of p16 protein.

关 键 词：人乳头状瘤病毒 宫颈腺癌 鳞状上皮内瘤变 P16蛋白 

分 类 号：R373[医药卫生—病原生物学]