玉米弯孢叶斑病菌REMI白化突变体的筛选与致病性测定  

Bending Spore of Corn Leaf Spot Fungus REMI Albino Mutant Screening and Determination of Pathogenic Transformation

作　　者：夏淑春[1] 王学武 张茹琴[1] 鄢洪海[1] 

机构地区：[1]青岛农业大学农学与植物保护学院,青岛266109 [2]青岛市植物保护站,青岛266100

出　　处：《生物技术通报》2014年第12期111-116,共6页Biotechnology Bulletin

基　　金：山东省科技发展项目(2009GG10009022);山东省自然科学基金项目(ZR2011CL005);山东省"泰山学者"建设工程专项经费资助项目(BS2009NY040)

摘　　要：旨在探讨玉米弯孢叶斑病菌致病机制及遗传变异特性,利用限制性内切酶介导的基因整合技术(REMI)对Curvularia lunata进行了遗传转化,共获得109个稳定的突变株。PCR检测结果表明,质粒p UC-JS已成功导入突变株中,转化子既有单拷贝也有多拷贝,白化菌株单拷贝插入频率为40%。此外,白化玉米弯孢叶斑病菌REMI突变株菌落颜色与野生菌株差异明显,并且在致病性上也明显弱于野生菌株;质粒拯救、测序与blast分析推测插入可能导致FAD3基因功能丧失。To investigate bending spore corn leaf spot fungus pathogenic mechanism and genetic variation characteristics, this study using restriction enzymes mediated gene integration technology （ REMI ） of Curvularia lunata genetic transformation, received 109 stable mutant strains. PCR detection results showed that the plasmid pUC-JS has successfully imported mutant strains, into the son has both single copy and copy, albino strain single copy insert frequency was 40%. In addition, an albino corn bending spore bacteria leaf spot REMI contrast with wild strain of the mutant strains colony color, and also significantly weaker than wild on pathogenic strains. Plasmid save, sequencing and analysis of blast speculated that inserts may result in the loss FAD3 gene function.

关 键 词：玉米弯孢菌叶斑病菌 REMI 白化菌株 致病性 

分 类 号：S435.13[农业科学—农业昆虫与害虫防治]