印度梨形孢定殖油菜对萝卜蚜选择油菜寄主的影响  

Influence of Piriformospora indica on Host Plant Selection by Aphid Lipaphis erysimi(Kaltenbach)

作　　者：刘金华[1] 王婷[1] 高其康[1] 

机构地区：[1]浙江大学昆虫科学研究所,杭州310058

出　　处：《生物技术通报》2014年第12期133-140,共8页Biotechnology Bulletin

摘　　要：旨在研究印度梨形孢(Piriformospora indica)定殖油菜(Brassica napus L.)对萝卜蚜(Lipaphis erysimi(Kaltenbach))选择寄主油菜的影响,分别在大棚拟自然环境和实验室人工环境下进行蚜虫双向选择试验,发现萝卜蚜在有印度梨形孢定殖油菜上的数量显著低于无印度梨形孢定殖的油菜。利用顶空动态吸附装置收集不同处理油菜的挥发性物质,通过气相色谱-质谱联用仪(GC-MS)分析收集到的两者挥发物在种类和含量上的差异。结果表明,有印度梨形孢定殖的油菜与无印度梨形孢定殖的油菜,挥发物的种类没有变化,而挥发物的总量、α-pinene、terpinolene、camphor、β-thujene、cedrene以及β-caryophyllene,在单位时间内有印度梨形孢定殖的油菜释放的量显著高于无印度梨形孢定殖的油菜。通过四扇区嗅觉仪测定萝卜蚜对挥发物的生物反应,发现α-pinene对萝卜蚜有显著的吸引作用,而camphor以及camphor与α-pinene浓度比为2∶1混合物对萝卜蚜具有显著的驱避作用,其余物质则没有作用。In order to confirm the influence of Piriformospora indica on host plant selection of L. erysimi. Aphid dual-choice assay was taken under greenhouse and artificial control environment and the amounts of aphids on the P. indica-colonized B. napus was significantly less than the P. indica-free B. napus. Volatiles emitted by plants collected by dynamic headspace systems, the quality and quantitative differences of volatiles emitted by B. napus were analyzed through GC-MS. The result indicated that P. indica inoculated B. napus and P. indica-free B. napus had the same valatiles, but the quantity of total volatiles, α-pinene, terpinolene, camphor, β-thujene, cedrene and β-caryophyllene emitted by B. napus with P. indica were significantly higher than P. indica-free B. napus in unit time. Through the four-sector offactomete method, we confirmed that theα-pinene significantly attracted the L. erysimi, while the camphor significantly repelled the L. erysimi. And the mixture of camphor and α-pinene （ 2 ： 1 ） was also significantly repelled the L erysimi. Terpinolene, β-thujene, cedrene and β-caryophyllene had no effect on the L erysimi.

关 键 词：印度梨形孢 萝卜蚜 寄主选择 挥发物 

分 类 号：S435.654[农业科学—农业昆虫与害虫防治]